抗Integrin αVβ3抗体(Anti-Integrin αVβ3, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Human osteoclasts
Source	Monoclonal Mouse IgG1 Clone # 23C6
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Integrin alpha V beta 3.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of Integrin  alpha V beta 3 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human Integrin  alpha V beta 3 PE‑conjugated Monoclonal Antibody (Catalog # FAB3050P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

Integrin alpha V beta 3

background_content

Background: Integrin alpha V beta 3 Integrin alpha V beta 3, together with Integrin  alpha IIb beta 3, constitute the only known beta 3 Integrins (1‑3). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N-terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta-propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alpha V subunit domains termed thigh, calf-1 and calf-2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alpha V beta 3 (4, 10). The 962 aa human alpha V ECD (11) shares 92‑95% aa sequence identity with mouse, rat and cow  alpha V while the 685 aa human beta 3 ECD (12) shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and pig beta 3.

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Citations

1. Evaluation of a dimeric-cRGD peptide for targeted PET-CT imaging of peripheral angiogenesis in diabetic mice
   Authors: J Hedhli, SLL Slania, A P?oska, A Czerwinski, CJ Konopka, M Wozniak, M Banach, IT Dobrucki, L Kalinowski, LW Dobrucki
   Sci Rep, 2018;8(1):5401.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Synthesis, Chemical Characterization and Multiscale Biological Evaluation of a Dimeric-cRGD Peptide for Targeted Imaging of ? V ? 3 Integrin Activity
   Authors: J Hedhli, A Czerwinski, M Schuelke, A P?oska, P Sowinski, L Hood, SB Mamer, JA Cole, P Czaplewska, M Banach, IT Dobrucki, L Kalinowski, P Imoukhuede, LW Dobrucki
   Sci Rep, 2017;7(1):3185.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer.
   Authors: Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E
   J. Exp. Clin. Cancer Res., 2012;31(0):26.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Combination of RGD compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells.
   Authors: Chang MW, Lo JM, Juan HF, Chang HY, Chuang CY
   PLoS ONE, 2012;7(5):e37935.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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