抗M-CSF Receptor抗体(Anti-M-CSF Receptor, Sheep-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse M-CSF R/CD115Ala20-Ser511Accession # P09581
Source	Polyclonal Sheep IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse M-CSF R/CD115 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant humanM-CSF R/CD115 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse M-CSF R/CD115 Fc Chimera (Catalog # 3818-MR)
Flow Cytometry	0.25 µg/106 cells	See below

Flow Cytometry
	Detection of M-CSF R/CD115 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse M-CSF R/CD115 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF3818, filled histogram) or control antibody (Catalog # BAF020, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

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Related Product & Information

Background

M-CSF R/CD115

background_content

Background: M-CSF R/CD115 M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Mouse M-CSF receptor cDNA encodes a 977 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 492 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 441 aa cytoplasmic domain. The mouse M-CSF R ECD shares >99% aa identity with rat and 60-63% aa identity with corresponding sequences in human, canine, feline and bovine M-CSF R. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation, and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts, and DC (3, 4). M-CSF R and integrin alpha v beta 3 share signaling pathways during osteoclastogenesis, and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

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Citations

1. HLA-DR7 and HLA-DQ2: Transgenic mouse strains tested as a model system for ximelagatran hepatotoxicity
   Authors: H Lundgren, K Martinsson, K Cederbrant, J Jirholt, D Mucs, K Madeyski-B, S Havarinasa, P Hultman
   PLoS ONE, 2017;12(9):e0184744.
   Species: Mouse
   Sample Type: Plasma
   Application: ELISA detection


2. Fos plays an essential role in the upregulation of RANK expression in osteoclast precursors within the bone microenvironment.
   Authors: Arai A, Mizoguchi T, Harada S, Kobayashi Y, Nakamichi Y, Yasuda H, Penninger J, Yamada K, Udagawa N, Takahashi N
   J Cell Sci, 2012;125(0):2910-7.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen

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