抗Canine HGF R/c-MET抗体(Anti-Canine HGF R/c-MET antibody)
掲載日情報：2021/01/28 現在Webページ番号：34263
Canine HGF R/c-METに対する抗体(Anti-Canine HGF R/c-MET )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年10月25日 13時35分現在]
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Anti-Canine HGF R/c-MET Affinity Purified Polyclonal Ab (100 UG) |
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Anti-Canine HGF R/c-MET Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2025年10月25日 13時35分現在]
Anti-Canine HGF R/c-MET Affinity Purified Polyclonal Ab (100 UG)
文献数： 2
- 商品コード：AF4140
- メーカー：RSD
- 包装：100μg
- 価格：¥78,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Canine HGF R/c-MET サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF4140，スタンダードとして#4140-ME-050を用いる。 別名：AUTS9 Genbank No： 4233 Protein Accession No： Q75ZY9 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Canine | 免疫動物 | Goat | ||||
| 交差性 | Canine | 適用 | Blocking,ELISA,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Canine HGF R/c-MET Affinity Purified Polyclonal Ab
文献数： 2
- 商品コード：AF4140-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥25,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：AUTS9 Genbank No： 4233 Protein Accession No： Q75ZY9 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Canine | 適用 | Blocking,ELISA,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Canine |
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| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant canine HGF R/c‑METGlu25-Leu935Accession # Q75ZY9 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects canine HGF R/c‑MET in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human HGF R and remombinant mouse HGF R is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Canine HGF R/c-MET (Catalog # 4140-ME) |
| Blockade of Receptor-ligand Interaction | In a functional ELISA, 1-5 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of biotinylated Recombinant Canine HGF to immobilized Recombinant Canine HGF R/c-MET (Catalog # 4140-ME) coated at 2 µg/mL (100 µL/well). At 25 μg/mL, this antibody will block >90% of the binding. | |
| Canine HGF R/c-MET Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Canine HGF R/c‑MET Antibody (Catalog #AF4140 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Canine HGF R/c-MET Biotinylated Antibody (Catalog #BAF4140 ) |
| ELISA Standard | Recombinant Canine HGF R/c-MET Protein, CF (Catalog #4140-ME ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: HGF R/c-MET | HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes posttranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS region, and four Ig-like E-set domains, while the cytoplasmic region includes a tyrosine kinase domain (3). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 4). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (5, 6). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (7). In the absence of ligand, HGF R forms noncovalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, integrin alpha 6/ beta 4, plexins B1, B2, and B3, and MSP R/Ron (8‑15). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (8‑15). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (8, 12, 13). HGF released from neighboring mesenchymal cells stimulates HGF R on undifferentiated epithelium and induces epithelial cell scattering and branching tubulogenesis (16). Genetic polymorphisms, chromosomal translocation, overexpression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, canine HGF R shares 85%‑88% amino acid sequence identity with human, mouse and rat HGF R.
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References
| Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915. | |
| Corso, S. et al. (2005) Trends Mol. Med. 11:284. | |
| Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039. | |
| Kong-Beltran, M. et al. (2004) Cancer Cell 6:75. | |
| Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793. | |
| Ponzetto, C. et al. (1994) Cell 77:261. | |
| Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799. | |
| Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074. | |
| Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408. | |
| Jo, M. et al. (2000) J. Biol. Chem. 275:8806. | |
| Wang, X. et al. (2002) Mol. Cell 9:411. | |
| Trusolino, L. et al. (2001) Cell 107:643. | |
| Giordano, S. et al. (2002) Nat. Cell Biol. 4:720. | |
| Conrotto, P. et al. (2004) Oncogene 23:5131. | |
| Follenzi, A. et al. (2000) Oncogene 19:3041. | |
| Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223. | |
| Long Name: | Hepatocyte Growth Factor Receptor |
| Entrez Gene IDs: | 4233 (Human); 17295 (Mouse) |
| Alternate Names: | AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF R; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met |
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Related Research Areas
| Cancer Biomarkers | ||
| Cancer Stem Cell Markers | ||
| Cytokine and Growth Factor Receptors on VSMC | ||
| Cytokines and Receptors in Angiogenesis | ||
| Hepatic Endoderm | ||
| Hepatic Endoderm Cell Markers | ||
| HIF Transcription Factors | ||
| Receptor Tyrosine Kinases (RTKs) | ||
| Receptor Tyrosine Kinases (RTKs) in the Akt Pathway | ||
| Receptors in the Jak/STAT Pathway | ||
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Related Product & Information
| Long Name | Hepatocyte Growth Factor Receptor |
|---|---|
| Entrez Gene IDs | 4233 (Human); 17295 (Mouse) |
| Background | HGF R/c-MET |
| background_content | Background: HGF R/c-MET HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes posttranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS region, and four Ig-like E-set domains, while the cytoplasmic region includes a tyrosine kinase domain (3). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 4). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (5, 6). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (7). In the absence of ligand, HGF R forms noncovalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, integrin alpha 6/ beta 4, plexins B1, B2, and B3, and MSP R/Ron (8‑15). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (8‑15). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (8, 12, 13). HGF released from neighboring mesenchymal cells stimulates HGF R on undifferentiated epithelium and induces epithelial cell scattering and branching tubulogenesis (16). Genetic polymorphisms, chromosomal translocation, overexpression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, canine HGF R shares 85%‑88% amino acid sequence identity with human, mouse and rat HGF R. |
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Citations
- Aberrant endocytosis leads to the loss of normal mitotic spindle orientation during epithelial glandular morphogenesis
Authors: JW Clancy, CS Sheehan, CJ Tricarico, C D'Souza-Sc
J. Biol. Chem., 2018;0(0):.
Species: Canine
Sample Type: Whole Cells
Application: ICC - Dorsal ruffle microdomains potentiate Met receptor tyrosine kinase signaling and down-regulation.
Authors: Abella JV, Parachoniak CA, Sangwan V, Park M
J. Biol. Chem., 2010;285(32):24956-67.
Species: Canine
Sample Type: Whole Cells
Application: ICC
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