抗Human/Rat CCR8 Phycoerythrin抗体(Anti-Human/Rat CCR8 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Rat
Label	Phycoerythrin
Immunogen	BaF3 mouse pro-B cell line transfected with human CCR8Met1-Leu355Accession # P51685
Source	Monoclonal Rat IgG2B Clone # 191704
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not with the parent cell lines.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of CCR8 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin  alpha M/CD11b Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB16991G) and either (A) Rat Anti-Human/Rat CCR8 PE‑conjugated Monoclonal Antibody (Catalog # FAB1429P) or (B) Rat IgG2B Phycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.
Flow Cytometry
	Detection of CCR8 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Rat Anti-Human/Rat CCR8 PE‑conjugated Monoclonal Antibody (Catalog # FAB1429P). Quadrant markers were set based on control antibody staining (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Entrez Gene IDs	1237 (Human); 12776 (Mouse)
Background	CCR8
background_content	Background: CCR8 CCR8 (C-C Chemokine Receptor 8), previously called CKR-L1 or TER1 and designated CD198, is a 41-43 kDa member of the GPCR 1 family of transmembrane proteins.  CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons.  CCL1/TCA3 and vMIP-1 are known agonists for CCR8.  Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in length.  It contains a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105 collectively, human and mouse CCR8 share 64% aa sequence identity.

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Citations

1. Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity
   Authors: Zaidoon Al-Jaderi
   Front Immunol, 2016;7(0):437.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
   Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
   J. Immunol., 2010;184(6):2886-98.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses.
   Authors: Carbone T, Nasorri F, Pennino D
   J. Immunol., 2010;184(2):1102-10.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Chemokine-mediated migration of skin-derived stem cells: predominant role for CCL5/RANTES.
   Authors: Kroeze KL, Jurgens WJ, Doulabi BZ, van Milligen FJ, Scheper RJ, Gibbs S
   J. Invest. Dermatol., 2009;129(6):1569-81.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
   Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D
   J. Immunol., 2007;179(6):4272-82.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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