抗IL-15抗体(Anti-IL-15, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	E. coli-derived recombinant human IL-15Asn49-Ser162Accession # P40933
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-15 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse IL-15 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL‑15 (Catalog # 247-IL)
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	Human peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin

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Related Product & Information

Background

IL-15

background_content

Background: IL-15 Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells.IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms ofIL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 amino acid (aa) protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain, and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

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Citations

1. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
   Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
   Blood, 2007;109(10):4272-9.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


2. Renal cells express a functional interleukin-15 receptor.
   Authors: Tejman-Yarden N, Zlotnik M, Lewis E, Etzion O, Chaimovitz C, Douvdevani A
   Nephrol. Dial. Transplant., 2005;20(3):516-23.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: DB


3. IL-21 sustains CD28 expression on IL-15-activated human naive CD8+ T cells.
   Authors: Alves NL, Arosa FA, van Lier RA
   J. Immunol., 2005;175(2):755-62.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray.
   Authors: Chen DS, Soen Y, Stuge TB
   PLoS Med., 2005;2(10):e265.
   Species: Human
   Sample Type: Whole Cells
   Application: functional microarray

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