抗IL-15抗体(Anti-IL-15, Human, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	E. coli-derived recombinant human IL-15Asn49-Ser162Accession # P40933
Source	Monoclonal Mouse IgG1 Clone # 34559
Purification	Protein A or G purified from ascites
Specificity	Detects human IL-15 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2, recombinant mouse IL-15, or rhIL-21 is observed.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of IL-15 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with LPS were stained with Mouse Anti-Human IL-15 PE-conjugated Monoclonal Antibody (Catalog # IC2471P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background

IL-15

background_content

Background: IL-15 Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1‑3). Mature human IL‑15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11‑13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

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Citations

1. Impaired TGF-? signaling in patients with active systemic lupus erythematosus is associated with an overexpression of IL-22
   Authors: R Rekik, M Smiti Khan, T Larbi, I Zamali, A Beldi-Ferc, O Kammoun, S Marzouki, S Hamzaoui, S Mrad, MR Barbouche, MH Houman, M Ben Ahmed
   Cytokine, 2018;108(0):182-189.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Interleukin-15 Dendritic Cells Harness NK Cell Cytotoxic Effector Function in a Contact- and IL-15-Dependent Manner.
   Authors: Anguille S, Van Acker H, Van den Bergh J, Willemen Y, Goossens H, Van Tendeloo V, Smits E, Berneman Z, Lion E
   PLoS ONE, 2015;10(5):e0123340.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration.
   Authors: Arneja A, Johnson H, Gabrovsek L, Lauffenburger D, White F
   J Immunol, 2014;192(1):123-35.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Interleukin-15 enhances cellular proliferation and upregulates CNS homing molecules in pre-B acute lymphoblastic leukemia.
   Authors: Williams M, Yousafzai Y, Cox C, Blair A, Carmody R, Sai S, Chapman K, McAndrew R, Thomas A, Spence A, Gibson B, Graham G, Halsey C
   Blood, 2014;123(20):3116-27.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. CD8+ T cells are activated in an antigen-independent manner in HIV-infected individuals.
   Authors: Bastidas S, Graw F, Smith M, Kuster H, Gunthard H, Oxenius A
   J Immunol, 2014;192(4):1732-44.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Survival and migration of human dendritic cells are regulated by an IFN-alpha-inducible Axl/Gas6 pathway.
   Authors: Scutera S, Fraone T, Musso T, Cappello P, Rossi S, Pierobon D, Orinska Z, Paus R, Bulfone-Paus S, Giovarelli M
   J. Immunol., 2009;183(5):3004-13.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients.
   Authors: Hussain SF, Kong LY, Jordan J, Conrad C, Madden T, Fokt I, Priebe W, Heimberger AB
   Cancer Res., 2007;67(20):9630-6.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs.
   Authors: Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Munz C
   Proc. Natl. Acad. Sci. U.S.A., 2004;101(47):16606-11.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. Dendritic Cell-Derived IL-32alpha: A Novel Inhibitory Cytokine of NK Cell Function.
   Authors: Gorvel L, Korenfeld D, Tung T, Klechevsky E
   J Immunol, 0;199(4):1290-1300.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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