抗IL-13抗体(Anti-IL-13, Human, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	E. coli-derived recombinant human IL-13Gly35-Asn146Accession # P35225
Source	Monoclonal Mouse IgG1 Clone # 32007
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-13 in flow cytometry.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of IL‑13 in NS0 Mouse Cell Line Transfected with Human IL-13 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human IL-13 was stained with Mouse Anti-Human IL‑13 PE‑conjugated Monoclonal Antibody (Catalog # IC2131P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background

IL-13

background_content

Background: IL-13 IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature human IL-13 shares 57%, 59%, and 94% amino acid sequence identity with mouse, rat, and rhesus macaque IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of pro-inflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is over-expressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

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Citations

1. A novel population of human CD56+ human leucocyte antigen D-related (HLA-DR+) colonic lamina propria cells is associated with inflammation in ulcerative colitis.
   Authors: Ng SC, Plamondon S, Al-Hassi HO, English N, Gellatly N, Kamm MA, Knight SC, Stagg AJ
   Clin. Exp. Immunol., 2009;158(2):205-18.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
   Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
   J. Immunol., 2008;180(7):5109-17.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. T-cell activation during exacerbations: a longitudinal study in refractory asthma.
   Authors: Mamessier E, Nieves A, Lorec AM, Dupuy P, Pinot D, Pinet C, Vervloet D, Magnan A
   Allergy, 2008;63(9):1202-10.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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