抗IL-15抗体(Anti-IL-15, Human, Mouse-Mono, FITC antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Fluorescein
Immunogen	E. coli-derived recombinant human IL-15Asn49-Ser162Accession # P40933
Source	Monoclonal Mouse IgG1 Clone # 34559
Purification	Protein A or G purified from ascites
Specificity	Detects human IL-15 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2, recombinant mouse IL-15, or rhIL-21 is observed.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of IL‑15 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with LPS were stained with Mouse Anti-Human IL‑15 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC2471F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background

IL-15

background_content

Background: IL-15 Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1‑3). Mature human IL‑15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11‑13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

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Citations

1. Phenotypic and Functional Characterization of NK Cells in Human Immune Response against the Dimorphic Fungus Paracoccidioides brasiliensis.
   Authors: Longhi LN, da Silva RM, Fornazim MC, Spago MC, de Oliveira RT, Nowill AE, Blotta MH, Mamoni RL
   J. Immunol., 2012;189(2):935-45.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Herpes simplex virus-1 up-regulates IL-15 gene expression in monocytic cells through the activation of protein tyrosine kinase and PKC zeta/lambda signaling pathways.
   Authors: Ahmad R, Ennaciri J, Cordeiro P, El Bassam S, Menezes J
   J. Mol. Biol., 2006;367(1):25-35.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
   Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
   J. Immunol., 2004;173(2):1463-76.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs.
   Authors: Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Munz C
   Proc. Natl. Acad. Sci. U.S.A., 2004;101(47):16606-11.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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