抗IL-12抗体(Anti-IL-12, Human, Mouse-Mono, FITC antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Fluorescein
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35Arg23-Ser219Accession # P29459
Source	Monoclonal Mouse IgG1 Clone # 27537
Purification	Protein A or G purified from ascites
Specificity	Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry
	Detection of IL‑12/IL‑35 p35 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC2191F) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
	Detection of IL‑12/IL‑35 p35 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC2191F) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background

IL-12/IL-35 p35

background_content

Background: IL-12/IL-35 p35 Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).

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Citations

1. B Lymphocytes in Multiple Sclerosis: Bregs and BTLA/CD272 Expressing-CD19+ Lymphocytes Modulate Disease Severity
   Sci Rep, 2016;6(0):29699.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis.
   Authors: Gironi, Maira, Saresella, Marina, Rovaris, Marco, Vaghi, Matilde, Nemni, Raffaell, Clerici, Mario, Grossi, Enzo
   Immun Ageing, 2013;10(1):1.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Vitamin D receptor gene polymorphisms and HLA DRB1*04 cosegregation in Saudi type 2 diabetes patients.
   Authors: Al-Daghri NM, Al-Attas O, Alokail MS
   J. Immunol., 2012;188(3):1325-32.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients.
   Authors: Hussain SF, Kong LY, Jordan J, Conrad C, Madden T, Fokt I, Priebe W, Heimberger AB
   Cancer Res., 2007;67(20):9630-6.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells.
   Authors: Lenz P, Bacot SM, Frazier-Jessen MR, Feldman GM
   FEBS Lett., 2003;538(1):149-54.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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