抗Human IL-18 R beta/IL-1 R7 Phycoerythrin抗体(Anti-Human IL-18 R beta/IL-1 R7 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human IL‑18 R beta /IL‑1 R7Met1-Arg356Accession # O95256
Source	Monoclonal Mouse IgG2B Clone # 132029
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL‑18 R beta /IL‑1 R7 in direct ELISAs and Western blots. In direct ELISAs, 15% cross-reactivity with recombinant human (rh) IL‑1 RII is observed and 2% cross-reactivity with rhIL-1 R9 is observed. Also in direct ELISAs, no cross-reactivity  with rhIL-1 RI, rhIL‑1 RacP/IL‑1 R3, rhIL-1 R4, rhIL-18 R alpha /IL-1 R5, rhIL-1 R6, rhIL-1 R8, or recombinant mouse (rm) IL-18 R beta /IL-1 R7 is observed. In Western blots, approximately 100% cross-reactivity with rmIL-18 R beta is observed and no cross-reactivity with rhIL-18 R alpha /IL-1 R5, rhIL‑1 RacP/IL‑1 R3, rhIL-1 RI, rhIL-1 RII, or rhIL-1 Rrp2/IL-1 R6 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of IL‑18 R beta /IL‑1 R7 in KG‑1 Human Cell Line by Flow Cytometry. KG‑1 human acute myelogenous leukemia cell line was stained with Mouse Anti-Human IL‑18 R beta /IL‑1 R7 PE-conjugated Mono­clonal Antibody (Catalog # FAB118P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

IL-18 R beta/IL-1 R7

background_content

Background: IL-18 R beta/IL-1 R7 IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL‑18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL‑18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.

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Citations

1. Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
   Authors: Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N
   J Immunol, 2015;195(5):2141-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Interleukin-18 produced by bone marrow-derived stromal cells supports T-cell acute leukaemia progression.
   Authors: Uzan B, Poglio S, Gerby B, Wu C, Gross J, Armstrong F, Calvo J, Cahu X, Deswarte C, Dumont F, Passaro D, Besnard-Guerin C, Leblanc T, Baruchel A, Landman-Parker J, Ballerini P, Baud V, Ghysdael J, Baleydier F, Porteu F, Pflumio F
   EMBO Mol Med, 2014;6(6):821-34.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Increased expression of Interleukin-18 receptor in blood cells of subjects with Mild Cognitive Impairment and Alzheimer's disease.
   Cytokine, 2013;61(2):360-3.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Defective phosphorylation of interleukin-18 receptor beta causes impaired natural killer cell function in systemic-onset juvenile idiopathic arthritis.
   Authors: De Jager W, Vastert SJ, Beekman JM, Wulffraat NM, Kuis W, Coffer PJ, Prakken BJ
   Arthritis Rheum., 2009;60(9):2782-93.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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