抗Human IL-23 R抗体(Anti-Human IL-23 R antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human IL-23 RGly24-Ile354Accession # Q5VWK5
Source	Monoclonal Mouse IgG2B Clone # 218213
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-23 R in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) IL‑23 R.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below

Western Blot

Detection of Human IL-23 R by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Human IL‑23 R Monoclonal Antibody (Catalog # MAB14001) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-23 R at approximately 58 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Background

IL-23 R

background_content

Background: IL-23 R Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 aa type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5. IL-23 has biological activities that are similar to, but distinct from IL-12.

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Citations

1. The human IL-23 receptor rs11209026 A allele promotes the expression of a soluble IL-23R-encoding mRNA species.
   Authors: Yu R, Brazaitis J, Gallagher G
   J Immunol, 2015;194(3):1062-8.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


2. Characterization of human CD39+ Th17 cells with suppressor activity and modulation in inflammatory bowel disease.
   Authors: Longhi M, Moss A, Bai A, Wu Y, Huang H, Cheifetz A, Quintana F, Robson S
   PLoS ONE, 2014;9(2):e87956.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Blocking of alpha4beta7 gut-homing integrin during acute infection leads to decreased plasma and gastrointestinal tissue viral loads in simian immunodeficiency virus-infected rhesus macaques.
   Authors: Ansari AA, Reimann KA, Mayne AE, Takahashi Y, Stephenson ST, Wang R, Wang X, Li J, Price AA, Little DM, Zaidi M, Lyles R, Villinger F
   J. Immunol., 2011;186(2):1044-59.
   Species: Primate - Macaca mulatta (Rhesus Macaque)
   Sample Type: Whole Cells
   Application: Flow


4. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
   Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
   Immunol. Lett., 2011;134(2):167-73.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. A naturally occurring, soluble antagonist of human IL-23 inhibits the development and in vitro function of human Th17 cells.
   Authors: Yu RY, Gallagher G
   J. Immunol., 2010;185(12):7302-8.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


6. Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
   Authors: Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
   J. Immunol., 2008;181(10):7420-7.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen

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