抗E-Cadherin抗体(Anti-E-Cadherin, Rat-Mono antibody)
掲載日情報：2021/01/28 現在Webページ番号：33583
E-Cadherinに対する抗体(Anti-E-Cadherin, Rat-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 ：2024年05月06日 00時00分現在]
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Anti-E-Cadherin, Rat-Mono(114403) |
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Anti-Mouse E-Cadherin MAb (Clone 114403) |
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[在庫・価格 ：2024年05月06日 00時00分現在]
Anti-E-Cadherin, Rat-Mono(114403)
文献数： 0
- 商品コード：MAB7482
- メーカー：RSD
- 包装：500μg
- 価格：¥122,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Mouse E-Cadherin サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF748，スタンダードとして#748-EC-050を用いる。 別名：Arc-1 クローン：114403 Genbank No： 999 Protein Accession No： P09803 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Mouse | 免疫動物 | Rat | ||||
| 交差性 | Mouse | 適用 | ELISA | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
抗幹細胞マーカー抗体／抗胚性幹細胞マーカー抗体 |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse E-Cadherin MAb (Clone 114403)
文献数： 0
- 商品コード：MAB7482-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 別名：Arc-1 クローン：114403 Genbank No： 999 Protein Accession No： P09803 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Mouse | 適用 | ELISA | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
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| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse E-CadherinAsp157-Val709 (predicted)Accession # P09803 |
| Source | Monoclonal Rat IgG1 Clone # 114403 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects mouse E-Cadherin in ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) VE-Cadherin, rmP-Cadherin, recombinant human (rh) Cadherin-17, or rhN-Cadherin is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Mouse E-Cadherin Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse E‑Cadherin Antibody (Catalog #MAB7482 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse E‑Cadherin Biotinylated Antibody (Catalog #BAF748 ) |
| ELISA Standard | Recombinant Mouse E-Cadherin Fc Chimera Protein, CF (Catalog #748-EC ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: E-Cadherin | Epithelial (E)‑Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium‑dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E‑Cadherin may also play a role in tumor development, as loss of E‑Cadherin has been associated with tumor invasiveness. E‑Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DxNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each. |
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References
| Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123. | |
| Overduin, M. et al. (1995) Science 267:386. | |
| Takeichi, M. (1991) 251:1451. | |
| Entrez Gene IDs: | 999 (Human); 12550 (Mouse); 83502 (Rat) |
| Alternate Names: | Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin |
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Related Research Areas
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Related Product & Information
| Background | E-Cadherin |
|---|---|
| background_content | Background: E-Cadherin Epithelial (E)‑Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium‑dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E‑Cadherin may also play a role in tumor development, as loss of E‑Cadherin has been associated with tumor invasiveness. E‑Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DxNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each. |
追加しました。
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