抗IgG 二次抗体(Anti-IgG, Mouse, Goat-Poly, FITC, F2 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Fluorescein
Immunogen	Mouse IgG heavy and light chains
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of mouse origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.

Flow Cytometry
	Detection of MouseIgG Primary Antibody by Flow Cytometry HT1080 humanfibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha V/CD51Monoclonal Antibody (Catalog # MAB1219,filled histogram) or isotype control antibody (Catalog # MAB002,open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG SecondaryAntibody (Catalog # F0103B).

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Related Product & Information

Long Name	Immunoglobulin G
Background	IgG
background_content	Background: IgG R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

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Citations

1. Tacrolimus Protects Podocytes from Injury in Lupus Nephritis Partly by Stabilizing the Cytoskeleton and Inhibiting Podocyte Apoptosis.
   Authors: Liao R, Liu Q, Zheng Z, Fan J, Peng W, Kong Q, He H, Yang S, Chen W, Tang X, Yu X
   PLoS ONE, 2015;10(7):e0132724.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


2. Quantification of endothelial microparticles on modified cytometric bead assay and prognosis in chest pain patients.
   Authors: Fan, Yuqi, Wang, Lianshen, Li, Yuanmin, Yin, Zhaofang, Xu, Zuojun, Wang, Changqia
   Circ J, 2014;78(1):206-14.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
   Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
   BMC Cancer, 2014;14(0):118.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. The role of RGD-tagged helical rosette nanotubes in the induction of inflammation and apoptosis in human lung adenocarcinoma cells through the P38 MAPK pathway.
   Authors: Suri SS, Rakotondradany F, Myles AJ, Fenniri H, Singh B
   Biomaterials, 2009;30(17):3084-90.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
   Authors: Ebert EC, Mehta V
   Cell. Immunol., 2006;241(1):7-13.
   Species: Human
   Sample Type: Whole Cells
   Application: Secondary

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