抗IFN-γ Receptor 1抗体(Anti-IFN-γ Receptor 1, Human, Mouse-Mono antibody)

• 2025/12/01

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  [RSD]厳選 抗体＆リコンビナントタンパク質 30％OFFキャンペーン[～2026/02/27] NEW

  期間：2025/12/01 ～2026/02/27

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  R&D Systems, Inc.（RSD）

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human IFN-gamma  R1Glu18-Gly245Accession # P15260.1
Source	Monoclonal Mouse IgG1 Clone # 92101
Purification	Protein A or G purified from ascites
Specificity	Detects human IFN-gamma R1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse IFN‑ gamma  R1, recombinant human (rh) IFN-gamma R2, or rhIL-10 R beta.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IFN-gamma  R1/CD119 (Catalog # 673-IR)
Flow Cytometry	2.5 µg/106 cells	Human whole blood monocytes
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize IFN‑ gamma  R1/CD119-mediated inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 0.01-0.03 µg/mL in the presence of 2 ng/mL Recombinant Human IFN‑ gamma.

Neutralization

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by HumanIFN‑ gamma  R1/CD119 Antibody. Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Anti-Human IFN‑ gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Human IFN‑ gamma  R1/CD119 Monoclonal Antibody (Catalog # MAB6731). The ND50 is typically 0.01-0.03 µg/mL.

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Related Product & Information

Long Name	Interferon gamma Receptor 1
Entrez Gene IDs	3459 (Human); 15979 (Mouse)
Background	IFN-gamma R1/CD119
background_content	Background: IFN-gamma R1/CD119 The high-affinity IFN-gamma  receptor complex is made up of two type I membrane proteins, IFN-gamma  R1 (IFN-gamma  R alpha ) and IFN-gamma  R2 (IFN-gamma  R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma  R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma  R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma  R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma  R1 share 52% amino acid sequence identity and bind IFN-gamma in a species-specific manner. IFN-gamma  R1 is constitutively expressed in most cell types. Soluble IFN-gamma  R1 that binds IFN-gamma has been detected in biological fluids.

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Citations

1. IL-1? induced HIF-1? inhibits the differentiation of human FOXP3(+) T cells
   Authors: LM Feldhoff, CM Rueda, ME Moreno-Fer, J Sauer, CM Jackson, CA Chougnet, J Rupp
   Sci Rep, 2017;7(1):465.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Bispecific T cell engager (BiTE�) antibody constructs can mediate bystander tumor cell killing
   Authors: SL Ross, M Sherman, PL McElroy, JA Lofgren, G Moody, PA Baeuerle, A Coxon, T Arvedson
   PLoS ONE, 2017;12(8):e0183390.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Induction of indoleamine 2,3-dioxygenase by Borrelia burgdorferi in human immune cells correlates with pathogenic potential.
   Authors: Love A, Schwartz I, Petzke M
   J Leukoc Biol, 2015;97(2):379-90.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Interferon-gamma differentially regulates TGF-beta1 and TGF-beta2 expression in human retinal pigment epithelial cells through JAK-STAT pathway.
   Authors: Nagineni CN, Cherukuri KS, Kutty V, Detrick B, Hooks JJ
   J. Cell. Physiol., 2007;210(1):192-200.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
   Authors: Ebert EC, Mehta V
   Cell. Immunol., 2006;241(1):7-13.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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