抗c-Myc抗体(Anti-c-Myc, Goat-Poly antibody)
掲載日情報：2018/11/26 現在Webページ番号：33263
c-Mycに対する抗体(Anti-c-Myc, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月07日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-c-Myc, Goat-Poly |
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Anti-Human c-Myc Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2024年05月07日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-c-Myc, Goat-Poly
文献数： 3
- 商品コード：AF3696
- メーカー：RSD
- 包装：100μg
- 価格：¥105,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
別名：avian myelocytomatosis viral oncogene homolog Genbank No： 4609 Protein Accession No： P01106 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Goat | ||||
| 交差性 | Human | 適用 | ChIP,IC,IHC,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
免疫染色システム ImmPRESS® Reagent Anti-Goat IgG R&D Systems 社 抗c-Myc抗体 |
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| 関連記事 | |||||||
Anti-Human c-Myc Affinity Purified Polyclonal Ab
文献数： 1
- 商品コード：AF3696-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 別名：avian myelocytomatosis viral oncogene homolog Genbank No： 4609 Protein Accession No： P01106 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Human | 適用 | ChIP,IC,IHC,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
免疫染色システム ImmPRESS® Reagent Anti-Goat IgG |
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| 関連記事 | |||||||
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Product Details
| Species Reactivity | Human, Mouse |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human c-Myc Arg66-Asp201Accession # P01106 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human c-Myc in direct ELISAs. Detects human and mouse c-Myc in Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) L-Myc and rhN-Myc is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.5 µg/mL | See below |
| Simple Western | 20 µg/mL | See below |
| Immunohistochemistry | 5-15 µg/mL | Immersion fixed paraffin-embedded sections of human liver cancer tissue |
| Chromatin Immunoprecipitation (ChIP) | 5 µg/5 x 106 cells | See below |
| Immunocytochemistry | 5-15 µg/mL | See below |
| Knockout Validated | c‑Myc is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in c‑Myc knockout HEK293T cell line. | |
| Western Blot | |
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 | Detection of Human c-Myc by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse c-Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for c-Myc at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Western Blot | |
 | Detection of Mouse c‑Myc by Western Blot. Western blot shows lysates of BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse c‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for c‑Myc at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Chromatin Immunoprecipitation (ChIP) | |
 | Detection of c‑Myc-regulated Genes by Chromatin Immunoprecipitation. HeLa human cervical epithelial carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. c‑Myc/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse c‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR. |
| Immunocytochemistry | |
 | c‑Myc in D3 Mouse Stem Cells. c‑Myc was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/Mouse c‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Immunocytochemistry | |
 | c‑Myc in BG01V Human Stem Cells. c‑Myc was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human/Mouse c‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Simple Western | |
 | Detection of Human c‑Myc by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Myc at approximately 78 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse c‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
| Knockout Validated | |
 | Western Blot Shows Human c‑Myc Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and c-Myc knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse c‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for c‑Myc at approximately 52 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Related Product & Information
| Long Name | v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian) |
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| Background | c-Myc |
| background_content | Background: c-Myc Human c-Myc is a 439 amino acid transcription factor with a bHLH/LZ (basic Helix-Loop-Helix, Leucine Zipper) domain. c-Myc DNA-binding and transcription function is achieved upon heterodimerization with its partner Max. c-Myc is often over-expressed and mutated in hematopoietic tumors. Mutations frequently result in truncations that remove the transactivation region or in the bHLH/LZ domain required for association with Max and DNA. Over the region used as immunogen, human c-Myc is 92% identical to the rat and mouse c-Myc proteins. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- Convergence of cMyc and beta-catenin on Tcf7l1 enables endoderm specification.
Authors: Morrison G, Scognamiglio R, Trumpp A, Smith A
EMBO J, 2015;35(3):356-68.
Species: Mouse
Sample Type: Cell Lysates
Application: ChIP
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