抗Human Endoglin/CD105 Phycoerythrin抗体(Anti-Human Endoglin/CD105 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Endoglin/CD105Glu26-Gly586Accession # Q5T9B9
Source	Monoclonal Mouse IgG1 Clone # 166707
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Endoglin/CD105.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of Endoglin/CD105 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Endoglin/CD105 PE-conjugated Mono­clonal Antibody (Catalog # FAB10971P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

目次に戻る

Related Product & Information

Background

Endoglin/CD105

background_content

Background: Endoglin/CD105 Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1‑3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Human Endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1-3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The human Endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine and canine Endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2‑4, 12‑14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

目次に戻る

Citations

1. Effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients
   Authors: C Maroun-Eid, A Ortega-Her, J Modrego, M Abad-Cardi, JA García-Don, L Reinares, N Martell-Cl, D Gómez-Garr
   PLoS ONE, 2018;13(1):e0190494.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Cranioplasty with Adipose-Derived Stem Cells, Beta-Tricalcium Phosphate Granules and Supporting Mesh: Six-Year Clinical Follow-Up Results
   Authors: T Thesleff, K Lehtimäki, T Niskakanga, S Huovinen, B Mannerströ, S Miettinen, R Seppänen-K, J Öhman
   Stem Cells Transl Med, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Therapeutic microparticles functionalized with biomimetic cardiac stem cell membranes and secretome
   Authors: J Tang, D Shen, TG Caranasos, Z Wang, AC Vandergrif, TA Allen, MT Hensley, PU Dinh, J Cores, TS Li, J Zhang, Q Kan, K Cheng
   Nat Commun, 2017;8(0):13724.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Derivation of therapeutic lung spheroid cells from minimally invasive transbronchial pulmonary biopsies
   Authors: PC Dinh, J Cores, MT Hensley, AC Vandergrif, J Tang, TA Allen, TG Caranasos, KB Adler, LJ Lobo, K Cheng
   Respir. Res., 2017;18(1):132.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions
   Authors: M Patrikoski, MHC Lee, L Mäkinen, XM Ang, B Mannerströ, M Raghunath, S Miettinen
   Stem Cells Int, 2017;2017(0):6909163.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer
   Authors: PL Mok, SN Leow, AE Koh, HH Mohd Nizam, SL Ding, C Luu, R Ruhaslizan, HS Wong, WH Halim, MH Ng, RB Idrus, SR Chowdhury, CM Bastion, SK Subbiah, A Higuchi, AA Alarfaj, KY Then
   Int J Mol Sci, 2017;18(2):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Study of the microRNA expression profile of foreskin derived mesenchymal stromal cells following inflammation priming
   Authors: H Fayyad-Kaz, M Fayyad-Kaz, B Badran, D Bron, L Lagneaux, M Najar
   J Transl Med, 2017;15(1):10.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. The differentiation potential of gingival mesenchymal stem cells induced by apical tooth germ cell?conditioned medium
   Mol Med Rep, 2016;14(4):3565-72.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model
   Stem Cells Dev, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. The effect of pro-inflammatory cytokines on immunophenotype, differentiation capacity and immunomodulatory functions of human mesenchymal stem cells
    Authors: Arash Pourgholam
    Cytokine, 2016;85(0):51-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

目次に戻る