抗Desmin抗体(Anti-Desmin, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant human DesminVal261-Leu470Accession # P17661
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human and mouse Desmin.

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Applications and Data

	Recommended Concentration	Sample
Immunohistochemistry	5-15 µg/mL	See below

Immunohistochemistry
	Desmin in Human Heart. Desmin was detected in immersion fixed paraffin-embedded sections of human heart using 15 µg/mL Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	Desmin in Mouse Skeletal Muscle. Desmin was detected in immersion fixed frozen sections of mouse skeletal muscle using Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to z-lines. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Entrez Gene IDs	1674 (Human); 13346 (Mouse); 64362 (Rat)
Background	Desmin
background_content	Background: Desmin Desmin is a 53 kDa, muscle-specific molecule that belongs to the intermediate filament superfamily of cytoskeletal proteins. Cytoplasmic Desmin homopolymers form stable filamentous networks that stabilize lateral alignments of myofibrils. Human Desmin is 470 amino acid (aa) peptide. It contains an N-terminal head segment (aa 1-84), multiple coiled regions (aa 109-412), and a tail region (aa 413-470). Single amino acid substitutions and three-amino acid deletions are associated with cardio‑skeletal myopathies. Most mutations affect filament assembly. Desmin is a highly conserved protein. Over the region used as immunogen, human Desmin is 99% identical to the corresponding mouse, canine, and porcine protein sequences.

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Citations

1. Altered AKAP12 expression in portal fibroblasts and liver sinusoids mediates transition from hepatic fibrogenesis to fibrosis resolution
   Authors: HS Lee, J Choi, T Son, HJ Wee, SJ Bae, JH Seo, JH Park, SH Ryu, D Lee, MK Jang, E Yu, YH Chung, KW Kim
   Exp. Mol. Med., 2018;50(4):48.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


2. Aneurysm Severity is Increased by Combined Mmp-7 Deletion and N-cadherin Mimetic (EC4-Fc) Over-Expression
   Authors: CA Lyon, H Williams, R Bianco, SJ Simmonds, BA Brown, KS Wadey, FCT Smith, JL Johnson, SJ George
   Sci Rep, 2017;7(1):17342.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


3. Dual inhibition of Ang-2 and VEGF receptors normalizes tumor vasculature and prolongs survival in glioblastoma by altering macrophages
   Authors: TE Peterson, ND Kirkpatric, Y Huang, CT Farrar, KA Marijt, J Kloepper, M Datta, Z Amoozgar, G Seano, K Jung, WS Kamoun, T Vardam, M Snuderl, J Goveia, S Chatterjee, A Batista, A Muzikansky, CC Leow, L Xu, TT Batchelor, DG Duda, D Fukumura, RK Jain
   Proc Natl Acad Sci USA, 2016;113(16):4470-5.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


4. Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival
   Authors: J Kloepper, L Riedemann, Z Amoozgar, G Seano, K Susek, V Yu, N Dalvie, RL Amelung, M Datta, JW Song, V Askoxylaki, JW Taylor, C Lu-Emerson, A Batista, ND Kirkpatric, K Jung, M Snuderl, A Muzikansky, KG Stubenrauc, O Krieter, H Wakimoto, L Xu, LL Munn
   Proc Natl Acad Sci USA, 2016;113(16):4476-81.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


5. Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
   Authors: Riordan D, Varma S, West R, Brown P
   PLoS ONE, 2015;10(7):e0128975.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
   Authors: Maury Y, Come J, Piskorowski R, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S
   Nat Biotechnol, 2015;33(1):89-96.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC OCT-embedded


7. Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions.
   Authors: Durruthy-Durruthy, Jens, Briggs, Sharon F, Awe, Jason, Ramathal, Cyril Y, Karumbayaram, Saravana, Lee, Patrick, Heidmann, Julia D, Clark, Amander, Karakikes, Ioannis, Loh, Kyle M, Wu, Joseph C, Hoffman, Andrew R, Byrne, James, Reijo Pera, Renee A, Sebastiano, Vittorio
   PLoS ONE, 2014;9(4):e94231.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


8. The novel desmin mutant p.A120D impairs filament formation, prevents intercalated disk localization, and causes sudden cardiac death.
   Authors: Brodehl A, Dieding M, Klauke B, Dec E, Madaan S, Huang T, Gargus J, Fatima A, Saric T, Cakar H, Walhorn V, Tonsing K, Skrzipczyk T, Cebulla R, Gerdes D, Schulz U, Gummert J, Svendsen J, Olesen M, Anselmetti D, Christensen A, Kimonis V, Milting H
   Circ Cardiovasc Genet, 2013;6(6):615-23.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC Fresh


9. Dual color photoactivation localization microscopy of cardiomyopathy-associated desmin mutants.
   Authors: Brodehl A, Hedde PN, Dieding M, Fatima A, Walhorn V, Gayda S, Šarić T, Klauke B, Gummert J, Anselmetti D, Heilemann M, Nienhaus GU, Milting H
   J. Biol. Chem., 2012;287(19):16047-57.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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