抗Human IL-17 RA/IL-17 R Phycoerythrin抗体(Anti-Human IL-17 RA/IL-17 R Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human IL-17 RLeu33-Trp320Accession # Q96F46
Source	Monoclonal Mouse IgG1 Clone # 133617
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 R PE-conjugated Monoclonal Antibody (Catalog # FAB177P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

IL-17 RA/IL-17 R

background_content

Background: IL-17 RA/IL-17 R Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL-17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

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Citations

1. IL-17A Contributes to the Pathogenesis of Endometriosis by Triggering Proinflammatory Cytokines and Angiogenic Growth Factors.
   Authors: Ahn S, Edwards A, Singh S, Young S, Lessey B, Tayade C
   J Immunol, 2015;195(6):2591-600.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. IL-17 Enhances Chemotaxis of Primary Human B Cells during Asthma.
   Authors: Halwani, Rabih, Al-Kufaidy, Roua, Vazquez-Tello, Alejandr, Pureza, Mary Ang, BaHammam, Ahmed S, Al-Jahdali, Hamdan, Alnassar, Sami A, Hamid, Qutayba, Al-Muhsen, Saleh
   PLoS ONE, 2014;9(12):e114604.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. T cell-derived IL-22 amplifies IL-1beta-driven inflammation in human adipose tissue: relevance to obesity and type 2 diabetes.
   Authors: Dalmas E, Venteclef N, Caer C, Poitou C, Cremer I, Aron-Wisnewsky J, Lacroix-Desmazes S, Bayry J, Kaveri S, Clement K, Andre S, Guerre-Millo M
   Diabetes, 2014;63(6):1966-77.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. TH17 cytokines induce human airway smooth muscle cell migration.
   Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
   J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.
   Authors: Brucklacher-Waldert V, Steinbach K, Lioznov M, Kolster M, Holscher C, Tolosa E
   J. Immunol., 2009;183(9):5494-501.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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