抗IgG 二次抗体(Anti-IgG, Mouse, Goat-Poly, PE, F2 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	Mouse IgG heavy and light chains
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of mouse origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.

Flow Cytometry
	Detection of MouseIgG Primary Antibody by Flow Cytometry Human peripheral blood granulocytes were stained with MouseAnti-Human CD157 Monoclonal Antibody (Catalog # MAB47361,filled histogram) or isotype control antibody (Catalog # MAB002,open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG SecondaryAntibody (Catalog # F0102B) at10 μL/106 cells.

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Related Product & Information

Long Name	Immunoglobulin G
Background	IgG
background_content	Background: IgG R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

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Citations

1. Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain
   Authors: RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots
   PLoS ONE, 2017;12(10):e0186317.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Renal Transplant Patients Biopsied for Cause and Tested for C4d, DSA, and IgG Subclasses and C1q: Which Humoral Markers Improve Diagnosis and Outcomes?
   Authors: JC Cicciarell, NA Lemp, Y Chang, M Koss, K Hacke, N Kasahara, KM Burns, DI Min, R Naraghi, T Shah
   J Immunol Res, 2017;2017(0):1652931.
   Species: Human
   Sample Type: Serum
   Application: Luminex Development


3. Podocalyxin promotes proliferation and survival in mature B-cell non-Hodgkin lymphoma cells
   Authors: E Tamayo-Orb, L Amo, M Riñón, N Nieto, E Amutio, N Maruri, M Solaun, A Arrieta, S Larrucea
   Oncotarget, 2017;8(59):99722-99739.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. IL-6 trans-signaling is another pathway to upregulate Osteopontin
   Cytokine, 2016;90(0):88-95.
   Species: Human
   Sample Type: Whole Cells
   Application: Secondary


5. Crosstalk between mitochondrial ROS and depolarization in the potentiation of TRAIL-induced apoptosis in human tumor cells.
   Authors: Suzuki-Karasaki M, Ochiai T, Suzuki-Karasaki Y
   Int J Oncol, 2014;44(2):616-28.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.
   Authors: Gaudineau B, Fougere M, Guaddachi F, Lemoine F, de la Grange P, Jauliac S
   J Cell Sci, 2012;125(0):4475-86.
   


7. HER3 targeting of adenovirus by fiber modification increases infection of breast cancer cells in vitro, but not following intratumoral injection in mice.
   Authors: MacLeod S, Elgadi M, Bossi G, Sankar U, Pisio A, Agopsowicz K, Sharon D, Graham F, Hitt M
   Cancer Gene Ther, 2012;19(12):888-98.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow control


8. Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells.
   Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
   PLoS ONE, 2011;6(2):e16899.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
   Authors: Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
   Int. J. Cancer, 2009;125(1):212-21.
   


10. Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine.
    Authors: Tang KF, He CX, Zeng GL, Wu J, Song GB, Shi YS, Zhang WG, Huang AL, Steinle A, Ren H
    Biochem. Biophys. Res. Commun., 2008;370(4):578-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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