抗KLF4抗体(Anti-KLF4, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human KLF4Ala2-Phe470Accession # AAH29923
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human KLF4 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows less than 30% cross‑reactivity with recombinant mouse KLF4.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	25 µg/mL	See below
Immunohistochemistry	15 µg/mL	See below
Chromatin Immunoprecipitation (ChIP)	5 µg/5 x 106 cells	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human KLF4 by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line, SW480 human colorectal adenocarcinoma cell line, and HCT‑116 human colorectal carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for KLF4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Chromatin Immunoprecipitation (ChIP)
	Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The B2R promoter was detected by standard PCR.
Immunocytochemistry
	KLF4 in BG01V Human Stem Cells. KLF4 was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	KLF4 in Human Colon. KLF4 was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Human KLF4 by Simple WesternTM. Simple Western lane view shows lysates of HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for KLF4 at approximately 63 kDa (as indicated) using 25 µg/mL of Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

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Related Product & Information

Long Name	Kruppel-Like Factor 4
Background	KLF4
background_content	Background: KLF4 Human KLF4, also known as epithelial zinc finger protein (EZF), is a 53 kDa (470 aa) member of the kruppel C2H2-type zinc finger protein family. It contains three C2H2-type zinc fingers at the carboxyl terminus that preferentially bind to cis-DNA elements that are GC rich. KLF4 regulates the expression of target genes that are involved in different cellular functions. KLF4 is highly expressed in the epithelial cells of the skin and the gastrointestinal tract. Human and mouse KLF4 share 90% amino acid sequence identity.

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Citations

1. Data in support of sustained upregulation of adaptive redox homeostasis mechanisms caused by KRIT1 loss-of-function
   Authors: C Antognelli, E Trapani, S Delle Mona, A Perrelli, C Fornelli, F Retta, P Cassoni, VN Talesa, SF Retta
   Data Brief, 2018;16(0):929-938.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. KLF2 and KLF4 control endothelial identity and vascular integrity
   Authors: P Sangwung, G Zhou, L Nayak, ER Chan, S Kumar, DW Kang, R Zhang, X Liao, Y Lu, K Sugi, H Fujioka, H Shi, SD Lapping, CC Ghosh, SJ Higgins, SM Parikh, H Jo, MK Jain
   JCI Insight, 2017;2(4):e91700.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
   Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
   Oncotarget, 2017;8(17):28074-28092.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer
   Authors: P Wang, WW Wan, SL Xiong, H Feng, N Wu
   Cell Death Discov, 2017;3(0):16105.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Induced overexpression of Oct4A in human dental pulp cells enhances pluripotency and multilineage differentiation capability.
   Authors: Liu L, Wu L, Wei X, Ling J
   Stem Cells Dev, 2015;24(8):962-72.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC Fluorescence


6. Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
   Authors: Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G
   J Clin Invest, 2014;124(9):3960-74.
   


7. ZNF750 interacts with KLF4 and RCOR1, KDM1A, and CTBP1/2 chromatin regulators to repress epidermal progenitor genes and induce differentiation genes.
   Authors: Boxer L, Barajas B, Tao S, Zhang J, Khavari P
   Genes Dev, 2014;28(18):2013-26.
   Species: Human
   Sample Type: Cell Lysates
   Application: IP


8. KLF4 modulates expression of IL-6 in dendritic cells via both promoter activation and epigenetic modification.
   Authors: Rosenzweig, Jason M, Glenn, Justin D, Calabresi, Peter A, Whartenby, Katharin
   J Biol Chem, 2013;288(33):23868-74.
   Species: Human
   Sample Type: Cell Lysates
   Application: EMSA


9. Endogenous Purification Reveals GREB1 as a Key Estrogen Receptor Regulatory Factor.
   Authors: Mohammed H
   Oncogene, 2012;3(2):342-9.
   Species: Human
   Sample Type: Cell Lysates
   Application: ChIP


10. Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins.
    Authors: Angel M, Yanik MF,
    PLoS ONE, 2010;5(7):e11756.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

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