抗Flt-1抗体(Anti-Flt-1, Rat-Mono, APC antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Mouse
Label	Allophycocyanin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse VEGF R1/Flt‑1Ser27-Glu759Accession # P35969
Source	Monoclonal Rat IgG2B Clone # 141522
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse VEGF R1/Flt‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human VEGF R1, recombinant mouse (rm) VEGF R2, or rmVEGF R3 is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of VEGF R1/Flt‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VEGF R1/Flt‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB4711A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

目次に戻る

Related Product & Information

Background

VEGF R1/Flt-1

background_content

Background: VEGF R1/Flt-1 VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (VEGF R1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis. Full length mouse VEGF R1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGF R1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGF R1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGF R1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

目次に戻る

Citations

1. Antifibrotic role of vascular endothelial growth factor in pulmonary fibrosis
   Authors: LA Murray, DM Habiel, M Hohmann, A Camelo, H Shang, Y Zhou, AL Coelho, X Peng, M Gulati, B Crestani, MA Sleeman, T Mustelin, MW Moore, C Ryu, AD Osafo-Addo, JA Elias, CG Lee, B Hu, JD Herazo-May, DA Knight, CM Hogaboam, EL Herzog
   JCI Insight, 2017;2(16):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis.
   Authors: Coffelt S, Kersten K, Doornebal C, Weiden J, Vrijland K, Hau C, Verstegen N, Ciampricotti M, Hawinkels L, Jonkers J, de Visser K
   Nature, 2015;522(7556):345-8.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets.
   Authors: Weston W, Zayas J, Perez R, George J, Jurecic R
   Sci Rep, 2014;4(0):5199.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

目次に戻る