抗Flt-1抗体(Anti-Flt-1, Rat-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse VEGF R1/Flt‑1Ser27-Glu759Accession # P35969
Source	Monoclonal Rat IgG2B Clone # 141522
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse VEGF R1/Flt‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human VEGF R1, recombinant mouse (rm) VEGF R2, or rmVEGF R3 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of VEGF R1/Flt‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VEGF R1/Flt‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB4711P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

VEGF R1/Flt-1

background_content

Background: VEGF R1/Flt-1 VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (VEGF R1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis. Full length mouse VEGF R1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGF R1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGF R1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGF R1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

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Citations

1. Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression
   Authors: WW Xu, B Li, XY Guan, SK Chung, Y Wang, YL Yip, SY Law, KT Chan, NP Lee, KW Chan, LY Xu, EM Li, SW Tsao, QY He, AL Cheung
   Nat Commun, 2017;8(0):14399.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.
   Authors: Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, Niese B, Li Y, Li Y, Courreges M, Benencia F
   Exp Cell Res, 2014;323(1):7-27.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. VEGFR1-positive macrophages facilitate liver repair and sinusoidal reconstruction after hepatic ischemia/reperfusion injury.
   Authors: Ohkubo, Hirotoki, Ito, Yoshiya, Minamino, Tsutomu, Eshima, Koji, Kojo, Ken, Okizaki, Shin-ich, Hirata, Mitsuhir, Shibuya, Masabumi, Watanabe, Masahiko, Majima, Masataka
   PLoS ONE, 2014;9(8):e105533.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Expression of VEGF receptors on endothelial cells in mouse skeletal muscle.
   Authors: Imoukhuede, Princess, Popel, Aleksand
   PLoS ONE, 2012;7(9):e44791.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Bone marrow subsets differentiate into endothelial cells and pericytes contributing to Ewing's tumor vessels.
   Authors: Reddy K, Zhou Z, Schadler K
   Mol. Cancer Res., 2008;6(6):929-36.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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