抗Cadherin-13抗体(Anti-Cadherin-13, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Cadherin-13Glu23-Ala692Accession # P55290
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Cadherin-13 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant mouse Cadherin-13 is observed, and less than 10% cross-reactivity with recombinant human (rh) N-Cadherin is observed, and less than 2% cross-reactivity with rhCadherin-8, rhCadherin-11, rhCadherin-17, rhE-Cadherin, rhP-Cadherin, rhVE-Cadherin and rhR-Cadherin is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Flow Cytometry	2.5 µg/106 cells	NCI‑H460 human large cell lung carcinoma cell line
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human Cadherin‑13 by Western Blot. Western blot shows lysates of human brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Cadherin‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cadherin‑13 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunocytochemistry
	Cadherin‑13 in NCI-H460 Human Cell Line. Cadherin‑13 was detected in immersion fixed NCI-H460 human large cell lung carcinoma cell line using 10 µg/mL Goat Anti-Human Cadherin‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	Cadherin‑13 in Human Skin. Cadherin‑13 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Cadherin‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Human Cadherin‑13 by Simple WesternTM. Simple Western lane view shows lysates of human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for Cadherin‑13 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Cadherin‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3264) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

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Related Product & Information

Background

Cadherin-13

background_content

Background: Cadherin-13 Cadherin-13, also known as T-Cadherin and H-Cadherin, is a 105 kDa member of the cadherin family of transmembrane glycoproteins that mediate calcium-dependent intercellular adhesion (1). However, Cadherin-13 is an atypical member, lacking transmembrane and cytosolic domains and containing a GPI moiety that anchors Cadherin-13 to the plasma membrane (1‑2). Human Cadherin-13 is synthesized as a 713 amino acid (aa) precursor that contains a 22 aa signal sequence, a 116 aa propeptide, a 555 aa mature chain, and a second propeptide of 20 aa that is removed in the mature form to reveal the GPI anchor. The mature form contains five cadherin domains and eight potential sites for N-linked glycosylation. Mature human Cadherin-13 shares 96% aa identity with mature mouse Cadherin-13. Cadherin-13 is expressed in various tissues. It is highly expressed in the heart, and in the CNS, Cadherin-13 is expressed in the cerebral cortex, medulla, hippocampus, amygdala, thalamus, and substantia nigra (2). There are higher levels of Cadherin-13 in the adult brain than in developing brain (2). Cadherin‑13 is also expressed in skin in the basal layer of the epidermis, lung, liver, kidney, and blood vessels (2). The structural characteristics of Cadherin-13 predict that it is unlikely to function as a true adhesion molecule in vivo (2). It is suggested that it may act rather as a signaling receptor participating in recognition of the environment and regulation of cell motility, proliferation, and phenotype (2). Cellular expression levels of Cadherin‑13 in various tissues often correlate, negatively or positively, with the proliferative potential of the cells (2). Cadherin-13 may also act as a suppressor of tumor cell growth (2). This potential role for Cadherin‑13 was emphasized by localization of Cadherin-13 gene to chromosome 16q24, a region exhibiting loss of heterozygosity in many solid tumors (2). Allelic loss of chromosome bands 16q24.1-q24.2 and reduced expression of Cadherin‑13, as well as hypermethylation of the remaining allele have been detected in a considerable number of human cancers (2).

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Citations

1. Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release
   Authors: Y Obata, S Kita, Y Koyama, S Fukuda, H Takeda, M Takahashi, Y Fujishima, H Nagao, S Masuda, Y Tanaka, Y Nakamura, H Nishizawa, T Funahashi, B Ranscht, Y Izumi, T Bamba, E Fukusaki, R Hanayama, S Shimada, N Maeda, I Shimomura
   JCI Insight, 2018;3(8):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2
   Authors: S Fukuda, S Kita, Y Obata, Y Fujishima, H Nagao, S Masuda, Y Tanaka, H Nishizawa, T Funahashi, J Takagi, N Maeda, I Shimomura
   J. Biol. Chem, 2017;0(0):.
   Species: Mouse
   Sample Type: Serum
   Application: WB


3. Actin cytoskeleton regulates functional anchorage-migration switch during T-cadherin-induced phenotype modulation of vascular smooth muscle cells
   Authors: A Frismantie, E Kyriakakis, B Dasen, P Erne, TJ Resink, M Philippova
   Cell Adh Migr, 2017;0(0):1-17.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. T-Cadherin Expression in the Epidermis and Adnexal Structures of Normal Skin
   Authors: Stanislaw Buechner
   Dermatopathology (Basel), 2016;3(4):68-78.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


5. Vagal Nerve Stimulation Modifies Neuronal Activity and the Proteome of Excitatory Synapses of Amygdala/Piriform Cortex
   Authors: Georgia M Alexander
   J. Neurochem, 2016;0(0):.
   Species: Rat
   Sample Type: Protein
   Application: WB


6. T-cadherin is essential for adiponectin-mediated revascularization.
   Authors: Parker-Duffen, Jennifer, Nakamura, Kazuto, Silver, Marcy, Kikuchi, Ryosuke, Tigges, Ulrich, Yoshida, Sumiko, Denzel, Martin S, Ranscht, Barbara, Walsh, Kenneth
   J Biol Chem, 2013;288(34):24886-97.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


7. Cross-talk between EGFR and T-cadherin: EGFR activation promotes T-cadherin localization to intercellular contacts.
   Authors: Kyriakakis E, Maslova K, Frachet A, Ferri N, Contini A, Pfaff D, Erne P, Resink T, Philippova M
   Cell Signal, 2013;25(5):1044-53.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


8. Identification of proteins associating with glycosylphosphatidylinositol- anchored T-cadherin on the surface of vascular endothelial cells: role for Grp78/BiP in T-cadherin-dependent cell survival.
   Authors: Philippova M, Ivanov D, Joshi MB, Kyriakakis E, Rupp K, Afonyushkin T, Bochkov V, Erne P, Resink TJ
   Mol. Cell. Biol., 2008;28(12):4004-17.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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