抗Rhesus Macaque IL-18/IL-1F4抗体(Anti-Rhesus Macaque IL-18/IL-1F4 antibody)

掲載日情報:2019/10/21 現在Webページ番号:32367

Rhesus Macaque IL-18/IL-1F4に対する抗体(Anti-Rhesus Macaque IL-18/IL-1F4 )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2025年02月14日 19時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Rhesus Macaque IL-18/IL-1F4 Affinity Purified Polyclonal Ab (100 UG)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
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説明文
別名:Iboctadekin
Genbank No: 3606
Protein Accession No: AAK13416
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Rhesus Macaque 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,IC,Neutralising,Simple Western,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
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製品記事 M1/M2 Macrophage Activation Marker
Helper T Cells Marker Antibodies
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Anti-Rhesus Macaque IL-18/IL-1F4 Affinity Purified Polyclonal Ab
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
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  • お問い合わせ
説明文
※受注発注品。形状:溶液または凍結乾燥
別名:Iboctadekin
Genbank No: 3606
Protein Accession No: AAK13416
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,IC,Neutralising,Simple Western,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
Helper T Cells Marker Antibodies
使いっきり抗体
関連記事

[在庫・価格 :2025年02月14日 19時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Rhesus Macaque IL-18/IL-1F4 Affinity Purified Polyclonal Ab (100 UG)

文献数: 0

説明文 別名:Iboctadekin
Genbank No: 3606
Protein Accession No: AAK13416
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Rhesus Macaque 免疫動物 Goat
交差性 Human 適用 ELISA,IC,Neutralising,Simple Western,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
Helper T Cells Marker Antibodies
関連記事

Anti-Rhesus Macaque IL-18/IL-1F4 Affinity Purified Polyclonal Ab

文献数: 0

説明文 ※受注発注品。形状:溶液または凍結乾燥
別名:Iboctadekin
Genbank No: 3606
Protein Accession No: AAK13416
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat
交差性 Human 適用 ELISA,IC,Neutralising,Simple Western,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
Helper T Cells Marker Antibodies
使いっきり抗体
関連記事



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Product Details

Species ReactivityHuman, Rhesus Macaque
LabelUnconjugated
ImmunogenE. coli-derived recombinant rhesus macaque IL‑18/IL-1F4Tyr37-Asp193Accession # AAK13416
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects rhesus macaque IL‑18/IL-1F4 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse IL-18, recombinant rat IL-18, and recombinant porcine IL-18 is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot0.1 µg/mLRecombinant Rhesus Macaque IL‑18/IL‑1F4 (Catalog # 2548-RM)
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human IL‑18/IL‑1F4 Monoclonal Antibody (Catalog # MAB91242).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Total IL-18 DuoSet ELISA Kit (Catalog # DY318-05) for convenient development of a sandwich ELISA or the Human Total IL-18/IL-1F4 Quantikine ELISA Kit (Catalog # DL180) for a complete optimized ELISA.

Immunocytochemistry5-15 µg/mLSee below
Knockout Validated IL‑18/IL‑1F4 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in IL‑18/IL‑1F4 knockout HeLa cell line.
NeutralizationMeasured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line. Novick, D. et al. (1999) Immunity 10(1):127. The Neutralization Dose (ND50) is typically 0.4‑1.2 µg/mL in the presence of 10 ng/mL Recombinant Rhesus Macaque IL‑18/IL‑1F4 and 20 ng/mL Recombinant Human TNF‑ alpha.


Immunocytochemistry
IL‑18/IL‑1F4 in Human PBMCs.
IL‑18/IL‑1F4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human/Rhesus Macaque IL‑18/IL‑1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2548) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (green). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Neutralization
IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Primate IL‑18/IL‑1F4 Antibody.
In the presence of Recombinant Human TNF‑ alpha (20 ng/mL, Catalog # 210-TA), Recombinant Rhesus Macaque IL‑18/IL‑1F4 (Catalog # 2548-RM) stimulates IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN‑ gamma Quantikine ELISA Kit (Catalog # DIF50). Under these conditions, IFN‑ gamma secretion elicited by Recombinant Rhesus Macaque IL‑18/IL‑1F4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rhesus Macaque IL‑18/IL‑1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2548). The ND50 is typically 0.4-1.2 µg/mL.
Knockout Validated
Western Blot Shows Human IL‑18/IL‑1F4 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and IL-18/IL-1F4 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rhesus Macaque IL‑18/IL‑1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2548) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑18/IL‑1F4 at approximately 25 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.


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Related Product & Information

Long NameInterleukin 18
Entrez Gene IDs3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
BackgroundIL-18/IL-1F4
background_contentBackground:
IL-18/IL-1F4
Interleukin-18 (IL-18), also known as IL-1F4 and IFN-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines and is a key molecule in the innate immune response (1). Rhesus IL-18 is synthesized as a 24 kDa proprotein that contains a 36 amino acid (aa) propeptide and a 157 aa mature region (2). Under inflammatory conditions, the propeptide is cleaved by Caspase-1 in the cytoplasm to liberate the mature nonglycosylated 18 kDa monomeric IL-18 (3, 4). Mature rhesus IL-18 shares 96% aa sequence identity with human IL-18 and 60-76% with mouse, rat, canine, feline, and porcine IL-18. IL-18 is secreted by a variety of cell types including macrophages, dendritic cells, and epithelial cells (1, 5). Circulating mature IL-18 is sequestered by soluble IL-18 binding proteins (IL-18 BP) that inhibit IL-18 bioactivity (6). IL-18 interacts with the widely expressed IL-18 R alpha which then recruits the signaling subunit IL-18 R beta (7, 8). The IL-1 family member IL-1F7 also binds to IL-18 R alpha but does not recruit IL-18 R beta or induce signaling (9). IL-1F7 binds IL-18 BP and enhances its neutralizing effect on IL-18 activity (9). IL-18 synergizes with other cytokines to activate NK, Th1, and Th17 cells and to increase the production of IFN-gamma (1, 5, 10-12). IL-18 can also promote Th2 cytokine release which reduces the effectiveness of antiviral responses (13, 14). Increased levels of active IL-18 contribute to the severity of autoimmunity and hypertension, while deficiency of IL-18 results in symptoms of metabolic syndrome (1, 5, 15, 16). In cancer, IL-18 stimulates Th1 and NK cells to target tumor cells, but it can also promote angiogenesis, metastasis, and tumor cell immune evasion (11).


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