抗R-Spondin 1抗体(Anti-R-Spondin 1, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse R-Spondin 1 (R&D Systems, Catalog # 3474-RS)Ser21-Gly209Accession # Q9Z132
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse R-Spondin 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross‑reactivity with recombinant human R-Spondin 1 is observed and less than 5% cross-reactivity with recombinant mouse (rm) R-Spondin 3 and rmR-Spondin 4 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse R-Spondin 1 (Catalog # 3474-RS)
Immunohistochemistry	5-15 µg/mL	See below

Immunohistochemistry

R‑Spondin 1 in Mouse Ovary. R‑Spondin 1 was detected in immersion fixed frozen sections of E13.5 mouse ovary using Goat Anti-Mouse R‑Spondin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3474) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

R-Spondin 1

background_content

Background: R-Spondin 1 R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that belongs to the R-Spondin family (1, 2). R-Spondins share around 40% aa identity. All regulate Wnt/ beta -catenin signaling, but have distinct expression patterns (1 - 3). Like other R-spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (amino acids (aa) 34 - 135) followed by a thrombospondin (TSP-1) motif (aa 147 - 207) and a region rich in basic residues (aa 211 - 263). Only the furin-like domains are needed for beta -catenin stabilization (2, 4). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (5). R-Spondin 1 contains one potential N-glycosylation site. Over aa 21 - 209, mouse R-Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 5). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (6, 7). Disruption is also associated with palmoplantar keratosis (6, 7). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (8). Injection of recombinant R-Spondin 1 in mice causes activation of beta -catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (8, 9). R-Spondin 1 appears to regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptor, Kremen (10). This competition reduces internalization of DKK-1/LRP-6/Kremen complexes (10). Reports differ on whether R‑Spondin 1 binds LRP-6 directly (10 - 12).

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Citations

1. RSPOs facilitated HSC activation and promoted hepatic fibrogenesis
   Authors: X Yin, H Yi, L Wang, W Wu, X Wu, L Yu
   Oncotarget, 2016;7(39):63767-63778.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC paraffin embedded


2. R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis.
   Authors: Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, Catizone A, Ricci G
   PLoS ONE, 2015;10(4):e0124213.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. Bone marrow stromal cell transplantation mitigates radiation-induced gastrointestinal syndrome in mice.
   Authors: Saha S, Bhanja P, Kabarriti R
   PLoS ONE, 2012;6(9):e24072.
   Species: Mouse
   Sample Type: Serum
   Application: WB


4. R-spondin1 deficiency in mice improves glycaemic control in association with increased beta cell mass.
   Authors: Wong VS, Oh AH, Chassot AA, Chaboissier MC, Brubaker PL
   Diabetologia, 2011;54(7):1726-34.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


5. Human RSPO1/R-spondin1 is expressed during early ovary development and augments beta-catenin signaling.
   Authors: Tomaselli S, Megiorni F, Lin L, Mazzilli MC, Gerrelli D, Majore S, Grammatico P, Achermann JC
   PLoS ONE, 2011;6(1):e16366.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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