抗CEACAM-1抗体(Anti-CEACAM-1, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human CEACAM-1Gln35-Gly428Accession # P13688
Source	Monoclonal Mouse IgG2B Clone # 283340
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CEACAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) CD31, rhICAM-1, -2, -3, recombinant mouse MAdCAM-1, or rhVCAM-1.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of CEACAM‑1/CD66a in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human Fc gamma RIII (CD16) CFS-conjugated Monoclonal Antibody (Catalog # FAB2546F) and either (A) Mouse Anti-Human CEACAM‑1/CD66a PE‑conjugated Monoclonal Antibody (Catalog # FAB2244P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

CEACAM-1/CD66a

background_content

Background: CEACAM-1/CD66a Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM-1; also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I transmembrane proteins (CEACAM-1, 3, and 4) and GPI-linked molecules (CEACAM-5-8). There is no CEACAM-2 in human. The gene for human CEACAM-1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74 aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type Ig-like domain, followed by three C2-type Ig-like domains. It shows considerable glycosylation, including high mannose residues and (sialyl) LewisX (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1-3). In addition to the full length form, ten alternate splice forms have been reported (1, 4, 6-8). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V-type Ig-like domain (aa’s 35-142). The three soluble forms also contain the first two C2-type Ig-like domains (aa’s 145-317), with differences coming in the third C2-type Ig-like domain (6). The seven transmembrane isoforms are highly divergent. Five of the seven contain the V-type plus the first two C2-type domains and then diverge considerably both in the ECD and cytoplasmic region. The remaining two contain only the V‑type Ig-like domain, the transmembrane region, and either a full-length or truncated cytoplasmic tail (1, 8). The actual functions of the isoforms are unclear. Full-length mouse and rat CEACAM-1 are approximately 57% aa identical to human CEACAM-1; in the V‑type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium. It is known to act as an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEA and CEACAM-6/NCA (3, 9). On neutrophils, CEACAM-1 also binds to dendritic cell CD-SIGN via its LeX moiety, inducing dendritic cell maturation and a subsequent Th1-type response (10,11).

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Citations

1. Interferon regulatory factor 1 and a variant of heterogeneous nuclear ribonucleoprotein L coordinately silence the gene for adhesion protein CEACAM1
   Authors: KJ Dery, C Silver, L Yang, JE Shively
   J. Biol. Chem., 2018;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Establishment and Characterization of a Pair of Patient-derived Human Non-small Cell Lung Cancer Cell Lines from a Primary Tumor and Corresponding Lymph Node Metastasis
   Authors: F Gebauer, D Wicklein, M Tachezy, T Grob, D Steinemann, G Manukjan, G Göhring, B Schlegelbe, H Maar, JR Izbicki, M Bockhorn, U Schumacher
   Anticancer Res, 2016;36(4):1507-18.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Moraxella catarrhalis adhesin UspA1-derived recombinant fragment rD-7 induces monocyte differentiation to CD14+CD206+ phenotype.
   Authors: Xie, Qi, Brackenbury, Louise S, Hill, Darryl J, Williams, Neil A, Qu, Xun, Virji, Mumtaz
   PLoS ONE, 2014;9(3):e90999.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
   Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
   PLoS ONE, 2014;9(1):e84417.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. CEACAM1 regulates Fas-mediated apoptosis in Jurkat T-cells via its interaction with beta-catenin.
   Authors: Li Y, Shively J
   Exp Cell Res, 2013;319(8):1061-72.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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