抗MCP-1抗体(Anti-MCP-1, Mouse-Mono antibody)
掲載日情報：2021/01/28 現在Webページ番号：32162
MCP-1に対する抗体(Anti-MCP-1, Mouse-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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Anti-MCP-1, Mouse-Mono(280702) <Anti-CCL2> <Anti-JE> |
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Anti-Canine CCL2/JE/MCP-1 MAb (Clone 280702) |
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-MCP-1, Mouse-Mono(280702) <Anti-CCL2> <Anti-JE>
文献数： 0
- 商品コード：MAB28171
- メーカー：RSD
- 包装：500μg
- 価格：¥121,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Canine CCL2/JE/MCP-1 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF1774，スタンダードとして#1774-MC-025を用いる。 別名：C-C motif chemokine ligand 2 クローン：280702 Genbank No： 6347 Protein Accession No： P52203 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Canine | 免疫動物 | Mouse | ||||
| 交差性 | Canine | 適用 | ELISA,IC,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Canine CCL2/JE/MCP-1 MAb (Clone 280702)
文献数： 0
- 商品コード：MAB28171-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：C-C motif chemokine ligand 2 クローン：280702 Genbank No： 6347 Protein Accession No： P52203 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Canine | 適用 | ELISA,IC,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Canine |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant canine CCL2/JE/MCP-1Gln24-Pro101Accession # P52203 |
| Source | Monoclonal Mouse IgG1 Clone # 280702 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects canine CCL2/JE/MCP-1 in direct ELISAs and Western blots. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Canine CCL2/JE/MCP‑1 (Catalog # 1774-MC) |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Canine CCL2/JE/MCP-1 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Canine CCL2/JE/MCP‑1 Antibody (Catalog #MAB28171 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Canine CCL2/JE/MCP‑1 Biotinylated Antibody (Catalog #BAF1774 ) |
| ELISA Standard | Recombinant Canine CCL2/MCP-1 Protein (Catalog #1774-MC ) | |
| Neutralization | Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 300 ng/mL Recombinant Canine CCL2/JE/MCP‑1. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
![Neutralization CCL2/JE/MCP-1 Antibody (280702) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/CCL2_MAB28171_Block_Neutralize_9124.jpg) click image to view larger | Chemotaxis Induced by CCL2/JE/MCP‑1 and Neutralization by Canine CCL2/JE/MCP‑1 Antibody.Recombinant Canine CCL2/JE/MCP‑1 (Catalog # 1774-MC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine CCL2/JE/MCP‑1 (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB28171). The ND50 is typically 0.5-2.0 µg/mL. |
| Immunocytochemistry |
![Immunocytochemistry CCL2/JE/MCP-1 Antibody (280702) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/CCL2_MAB28171_Immunocytochemistry__Immunofluorescence_18502.jpg) click image to view larger | CCL2/JE/MCP‑1 in Canine PBMCs.CCL2/JE/MCP‑1 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB28171) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CCL2/JE/MCP-1 | Canine MCP-1 (monocyte chemotactic protein-1) is an 8 kDa member of the CC chemokine family of chemotactic factors (1, 2). It is synthesized as a 101 amino acid (aa) precursor that contains a 23 aa signal sequence and a 78 aa mature segment (3). It contains no potential N-linked glycosylation sites and is not known for any posttranslational modifications. Based on human studies, MCP-1 will primarily circulate as a monomer. Noncovalent dimers are likely to be found, however. MCP‑1 activity has been localized to the N-terminus (1). Cell types known to secrete MCP-1 are considerable in number, and include keratinocytes, fibroblasts, endothelium, osteoblasts, macrophages, mast cells, smooth muscle cells, and astrocytes (1, 2). In the mature MCP-1 segment, there is 82% and 83% aa identity, canine to human and porcine MCP-1, respectively. When mature canine MCP-1 is compared to (125 aa) extended rodent MCP-1, there is 55% and 56% aa identity, canine to mouse and rat MCP-1, respectively. MCP-1 has three possible receptors. The first two are CCR2 (1) and CCR11 (4). The third receptor has only been identified in mice and is called L-CCR (5). Its function is unknown. MCP-1 is best known as a chemotactic agent for mononuclear cells. It also, however, induces enzyme and cytokine release in monocytes, NK cells, and lymphocytes and histamine release by basophils (1). Additionally, it is believed to reduce IL-12 production by dendritic cells and promote a Th2 phenotype in CD4+ T cells (6). |
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References
| Coillie, E.V. et al. (1999) Cytokine Growth Factor Rev. 10:61. | |
| Yoshie, O. et al. (2001) Adv. Immunol. 78:57. | |
| Kumar, A.G. et al. (1997) Circulation 95:693. | |
| Biber, K. et al. (2003) J. Leukoc. Biol. 74:243. | |
| Luther, S.A. and J.G. Cyster (2001) Nat. Immunol. 2:102. | |
| Entrez Gene IDs: | 6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine) |
| Alternate Names: | C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF |
追加しました。
Related Research Areas
| Neutralization | |
|---|---|
 | Chemotaxis Induced by CCL2/JE/MCP‑1 and Neutralization by Canine CCL2/JE/MCP‑1 Antibody. Recombinant Canine CCL2/JE/MCP‑1 (Catalog # 1774-MC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine CCL2/JE/MCP‑1 (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB28171). The ND50 is typically0.5-2.0 µg/mL. |
| Immunocytochemistry | |
 | CCL2/JE/MCP‑1 in Canine PBMCs. CCL2/JE/MCP‑1 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB28171) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
追加しました。
Related Product & Information
| Entrez Gene IDs | 6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine) |
|---|---|
| Background | CCL2/JE/MCP-1 |
| background_content | Background: CCL2/JE/MCP-1 Canine MCP-1 (monocyte chemotactic protein-1) is an 8 kDa member of the CC chemokine family of chemotactic factors (1, 2). It is synthesized as a 101 amino acid (aa) precursor that contains a 23 aa signal sequence and a 78 aa mature segment (3). It contains no potential N-linked glycosylation sites and is not known for any posttranslational modifications. Based on human studies, MCP-1 will primarily circulate as a monomer. Noncovalent dimers are likely to be found, however. MCP‑1 activity has been localized to the N-terminus (1). Cell types known to secrete MCP-1 are considerable in number, and include keratinocytes, fibroblasts, endothelium, osteoblasts, macrophages, mast cells, smooth muscle cells, and astrocytes (1, 2). In the mature MCP-1 segment, there is 82% and 83% aa identity, canine to human and porcine MCP-1, respectively. When mature canine MCP-1 is compared to (125 aa) extended rodent MCP-1, there is 55% and 56% aa identity, canine to mouse and rat MCP-1, respectively. MCP-1 has three possible receptors. The first two are CCR2 (1) and CCR11 (4). The third receptor has only been identified in mice and is called L-CCR (5). Its function is unknown. MCP-1 is best known as a chemotactic agent for mononuclear cells. It also, however, induces enzyme and cytokine release in monocytes, NK cells, and lymphocytes and histamine release by basophils (1). Additionally, it is believed to reduce IL-12 production by dendritic cells and promote a Th2 phenotype in CD4+ T cells (6). |
追加しました。
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