抗H60抗体(Anti-H60, Mouse, Rat-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	BaF3 mouse pro-B cell line transfected with mouse H60 and mouse myeloma cell line NS0-derived recombinant mouse H60Asp30-Gln212Accession # Q3TDZ7
Source	Monoclonal Rat IgG2A Clone # 205326
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse H60 in ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of H60 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse H60 PE‑conjugated Monoclonal Antibody (Catalog # FAB1155P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

H60

background_content

Background: H60 H60 was originally described as an immunodominant histocompatibility antigen that is expressed in BALB mice but not in B6 mice. More recently it was reported to function as a ligand for mouse NKG2D, an activating receptor found on NK cells, on some T cell subsets, and on stimulated macrophages. H60 shares approximately 25 percent amino acid identity with the Rae-1 family, a small group of proteins that also function as ligands for mouse NKG2D. H60 and the Rae-1 proteins are distantly related to MHC class I proteins, but they possesses only the alpha 1 and alpha 2 Ig-like domains, and have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. Unlike the GPI-linked Rae-1 proteins, H60 appears to be anchored to the membrane via a hydrophobic transmembrane segment. H60 transcripts were found in embryonic tissue, in spleen, and in some transformed cell lines. Transcripts were also observed in mouse skin cells after exposure to carcinogens. Binding of H60 to NKG2D results in the activation of cytolytic activity and/or cytokine production by the NKG2D-expressing effector cells. Ectopic expression of H60 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1-6).

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Citations

1. Immunotherapy of Dual-Function Vector with Both Immunostimulatory and B-Cell Lymphoma 2 (Bcl-2)-Silencing Effects on Gastric Carcinoma
   Authors: L Ma, M Han, Z Keyoumu, H Wang, S Keyoumu
   Med. Sci. Monit., 2017;23(0):1980-1991.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Neural stem cells sustain natural killer cells that dictate recovery from brain inflammation.
   Authors: Liu Q, Sanai N, Jin W, La Cava A, Van Kaer L, Shi F
   Nat Neurosci, 2016;19(2):243-52.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. In vivo eradication of MLL/ENL leukemia cells by NK cells in the absence of adaptive immunity.
   Authors: Nakata J, Nakano K, Okumura A, Mizutani Y, Kinoshita H, Iwai M, Hasegawa K, Morimoto S, Fujiki F, Tatsumi N, Nakajima H, Nakae Y, Nishida S, Tsuboi A, Oji Y, Oka Y, Sugiyama H, Kumanogoh A, Hosen N
   Leukemia, 2014;28(6):1316-25.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Targeting Stat3 induces senescence in tumor cells and elicits prophylactic and therapeutic immune responses against breast cancer growth mediated by NK cells and CD4+ T cells.
   J. Immunol., 2012;189(3):1162-72.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Small intestinal intraepithelial lymphocytes expressing CD8 and T cell receptor gammadelta are involved in bacterial clearance during Salmonella enterica serovar Typhimurium infection.
   Authors: Li Z, Zhang C, Zhou Z, Zhang J, Zhang J, Tian Z
   Infect. Immun., 2012;80(2):565-74.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


6. NKG2D receptor signaling enhances cytolytic activity by virus-specific CD8+ T cells: evidence for a protective role in virus-induced encephalitis.
   Authors: Walsh KB, Lanier LL, Lane TE
   J. Virol., 2007;82(6):3031-44.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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