抗FABP4抗体(Anti-FABP4, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human FABP4/A‑FABPCys2-Ala132Accession # P15090
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human FABP4/A‑FABP in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse (rm) FABP4 is observed, approximately 30% cross‑reactivity with recombinant human (rh) FABP3 is observed, and less than 5% cross‑reactivity with rhFABP1, -2, -5, -6, -7, -8, -9, and rmFABP9 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human, Mouse, and Rat FABP4/A‑FABP by Western Blot. Western blot shows lysates of human heart tissue, rat heart tissue, mouse heart tissue, human adipose tissue, rat adipose tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FABP4/A‑FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	FABP4/A‑FABP in Human Bladder Cancer Tissue. FABP4/A‑FABP was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Human and Mouse FABP4/A‑FABP by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A‑FABP at approximately 19 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Fatty Acid-Binding Protein 4
Background	FABP4/A-FABP
background_content	Background: FABP4/A-FABP FABP4, also known as adipocyte P2 and A-FABP (adipocyte FABP), is a FABP family member that is expressed in adipocytes and monocyte-derived foam cells. It is a lipid transport protein that binds long chain fatty acid and retinoic acid. Human and mouse FABP4 share 91% amino acid sequence homology.

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Citations

1. Firemaster� 550 and its components isopropylated triphenyl phosphate and triphenyl phosphate enhance adipogenesis and transcriptional activity of peroxisome proliferator activated receptor (Ppar?) on the adipocyte protein 2 (aP2) promoter
   Authors: EWY Tung, S Ahmed, V Peshdary, E Atlas
   PLoS ONE, 2017;12(4):e0175855.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. Identification of multipotent stem cells in human brain tissue following stroke
   Authors: K Tatebayash, Y Tanaka, A Nakano-Doi, R Sakuma, S Kamachi, M Shirakawa, K Uchida, H Kageyama, T Takagi, S Yoshimura, T Matsuyama, T Nakagomi
   Stem Cells Dev, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Transcriptome sequencing wide functional analysis of human mesenchymal stem cells in response to TLR4 ligand
   Sci Rep, 2016;6(0):30311.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC - PFA fixed


4. Identification of a cell-of-origin for fibroblasts comprising the fibrotic reticulum in idiopathic pulmonary fibrosis.
   Authors: Xia H, Bodempudi V, Benyumov A, Hergert P, Tank D, Herrera J, Braziunas J, Larsson O, Parker M, Rossi D, Smith K, Peterson M, Limper A, Jessurun J, Connett J, Ingbar D, Phan S, Bitterman P, Henke C
   Am J Pathol, 2014;184(5):1369-83.
   Species: Human
   Sample Type: Whole Cells
   Application: IF


5. Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling.
   Authors: Reinhardt P, Glatza M, Hemmer K, Tsytsyura Y, Thiel C, Hoing S, Moritz S, Parga J, Wagner L, Bruder J, Wu G, Schmid B, Ropke A, Klingauf J, Schwamborn J, Gasser T, Scholer H, Sterneckert J
   PLoS ONE, 2013;8(3):e59252.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
   Authors: Klim JR, Li L, Wrighton PJ
   Nat. Methods, 2010;7(12):989-94.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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