抗Human Serpin E1/PAI-1抗体(Anti-Human Serpin E1/PAI-1 antibody)
掲載日情報：2021/01/28 現在Webページ番号：31894
Human Serpin E1/PAI-1に対する抗体(Anti-Human Serpin E1/PAI-1 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月27日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Human Serpin E1/PAI-1 MAb (Clone 242816) |
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Anti-Human Serpin E1/PAI-1 MAb (Clone 242816) |
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Anti-Human Serpin E1/PAI-1 MAb (Clone 242816) |
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[在庫・価格 ：2025年04月27日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human Serpin E1/PAI-1 MAb (Clone 242816)
文献数： 2
- 商品コード：MAB1786-100
- メーカー：RSD
- 包装：100μg
- 価格：¥101,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：Endothelial plasminogen activator inhibitor クローン：242816 Genbank No： 5054 Protein Accession No： P05121 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human Serpin E1/PAI-1 MAb (Clone 242816)
文献数： 2
- 商品コード：MAB1786-500
- メーカー：RSD
- 包装：500μg
- 価格：¥232,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human Serpin E1/PAI-1 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF1786，スタンダードとして#1786-PI-010を用いる。 別名：Endothelial plasminogen activator inhibitor クローン：242816 Genbank No： 5054 Protein Accession No： P05121 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | Quantikine Human / Total Human Serpin E1 (PAI-1) ELISA Kit |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human Serpin E1/PAI-1 MAb (Clone 242816)
文献数： 2
- 商品コード：MAB1786-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：Endothelial plasminogen activator inhibitor クローン：242816 Genbank No： 5054 Protein Accession No： P05121 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin E1/PAI-1Met1-Pro402Accession # P05121 |
| Source | Monoclonal Mouse IgG1 Clone # 242816 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human Serpin E1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Serpin A1, A3, A4, A8, C1, F1, F2, I1, I2, recombinant mouse Serpin D1 or E2 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | See below |
| Simple Western | 2.5 µg/mL | See below |
| Human Serpin E1/PAI-1 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human Serpin E1/PAI‑1 Antibody (Catalog #MAB1786 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human Serpin E1/PAI‑1 Biotinylated Antibody (Catalog #BAF1786 ) |
| ELISA Standard | Recombinant Human Serpin E1/PAI-1 Protein, CF (Catalog #1786-PI ) | |
| Neutralization | Measured by its ability to neutralize Recombinant Human Serpin E1/PAI-1 (0.25 µg/mL, Catalog # 1786-PI) inhibition of Recombinant Human u‑Plasminogen Activator (uPA)/Urokinase (0.1 µg/mL, Catalog # 1310-SE) cleavage of the fluorogenic peptide substrate Z-GGR-AMC (100 µM). The Neutralization Dose (ND50) is typically 0.3 µg/mL. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Western Blot |
![Western Blot Serpin E1/PAI-1 Antibody (242816) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/Serpin_E1_MAB1786_Western_Blot_20071.jpg) click image to view larger | Detection of Human Serpin E1/PAI‑1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin E1/PAI‑1 Monoclonal Antibody (Catalog # MAB1786) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin E1/PAI‑1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Neutralization |
![Neutralization Serpin E1/PAI-1 Antibody (242816) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/Serpin_E1_MAB1786_Block_Neutralize_10048.jpg) click image to view larger | Neutralization of Serpin E1/ PAI‑1 Activity by Human Serpin E1/PAI‑1 Antibody. Recombinant Human u‑Plasminogen Activator (uPA)/Urokinase (0.1 µg/mL, Catalog # 1310-SE) activity is measured in the presence of Recombinant Human Serpin E1/PAI-1 (0.25 µg/mL, Catalog # 1786-PI) that has been preincubated with increasing concentrations of Mouse Anti-Human Serpin E1/PAI‑1 Monoclonal Antibody (Catalog # MAB1786). The ND50 is typically 0.3 µg/mL. |
| Simple Western |
![Simple Western Serpin E1/PAI-1 Antibody (242816) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/Serpin_E1_MAB1786_Simple_Western_20086.jpg) click image to view larger | Detection of Human Serpin E1/PAI‑1 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI‑1 at approximately 54 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human Serpin E1/PAI‑1 Monoclonal Antibody (Catalog # MAB1786). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: Serpin E1/PAI-1 | As a member of the Serpin superfamily of serine protease inhibitors, Serpin E1/PAI‑1 is the principal inhibitor of urokinase‑type plasminogen activator (uPA) and tissue‑type PA (1, 2). As important regulators of extracellular matrix remodeling, uPA and PAI‑1 play a major role in many processes such as angiogenesis, tumor invasion and obesity (2‑4). For example, uPA and PAI-1 are the only tumor prognostic factors validated at the highest level of evidence with regard to their clinical utility in breast cancer (5). The human PAI-1 is initially synthesized as 402 amino acid precursor with a N‑terminal signal peptide (6, 7). PAI‑1 may exist in one of two possible conformations, designated as active or latent (8). The purified rhPAI‑1 is active against rhuPA. The heterogeneity at the N‑terminus of the purified recombinant human PAI‑1 has been observed before for both the recombinant and native proteins (9). |
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References
| Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293. | |
| Stefansson, S. et al. (2003) Curr. Pharm. Des. 9:1545. | |
| Duffy, M.J. (2002) Clin. Chem. 48:1194. | |
| Juhan-Vague, I. et al. (2003) J. Thromb. Haemost. 1:1575. | |
| Harbeck, N. et al. (2002) Clin. Breast Cancer 3:196. | |
| Pannekoek, H. et al. (1986) EMBO J. 5:2539. | |
| Ginsburg, D. et al. (1986) J. Clin. Invest. 78:1673. | |
| Wang, Z. et al. (1996) Biochemistry 35:16443. | |
| Stromqvist, M. et al. (1994) Protein Expr. Purif. 5:309. | |
| Long Name: | Plasminogen Activator Inhibitor |
| Entrez Gene IDs: | 5054 (Human); 18787 (Mouse) |
| Alternate Names: | Endothelial plasminogen activator inhibitor; Nexin; PAI1; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1 |
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Related Research Areas
| Adipocytokines | ||||||||
| Alpha Granule Soluble Molecules | ||||||||
| Cancer Biomarkers | ||||||||
| Coagulation | ||||||||
| Complement Pathway | ||||||||
| DNA Damage and Repair | ||||||||
| Enzymes Secreted by Endothelial Cells | ||||||||
| Mesenchymal Cells and EMT | ||||||||
| Plasmin/Plasminogen and Kallikrein/Kinin Systems | ||||||||
| Serine Proteases and Regulators | ||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||
|---|---|---|---|---|---|---|---|---|
| Excellent | Block/Neutralize | Human | Anonymous | 05/14/2018 | ||||
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Summary
| Application | Block/Neutralize |
| Sample Tested | Breast cancer cells |
| Species | Human |
| Other Experimental Details | We used this Serpin E1 neutralizing antibody (400 ng/mL) as part of an antibody cocktail containing several other antibodies to neutralize/block Serpin E1 present in the Conditioned Medium (CM) from BT549 Cells treated with Angiotensin II. CM (+/- Serpin E1 Neutralizing ab Cocktail) was used to further look at Endothelial (HUVEC) Cell migration using Incucyte Chemotaxis Assay |
| Western Blot | |
|---|---|
 | Detection of Human Serpin E1/PAI‑1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin E1/PAI‑1 Monoclonal Antibody (Catalog # MAB1786) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin E1/PAI‑1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Neutralization | |
 | Neutralization of Serpin E1/PAI‑1 Activity by Human Serpin E1/PAI‑1 Antibody. Recombinant Human u‑Plasminogen Activator (uPA)/Urokinase (0.1 µg/mL, Catalog # 1310-SE) activity is measured in the presence of Recombinant Human Serpin E1/PAI-1 (0.25 µg/mL, Catalog # 1786-PI) that has been preincubated with increasing concentrations of Mouse Anti-Human Serpin E1/PAI‑1 Monoclonal Antibody (Catalog # MAB1786). The ND50 is typically 0.3 µg/mL. |
| Simple Western | |
 | Detection of Human Serpin E1/PAI‑1 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI‑1 at approximately 54 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human Serpin E1/PAI‑1 Monoclonal Antibody (Catalog # MAB1786). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
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Related Product & Information
| Background | Serpin E1/PAI-1 |
|---|---|
| background_content | Background: Serpin E1/PAI-1 As a member of the Serpin superfamily of serine protease inhibitors, Serpin E1/PAI‑1 is the principal inhibitor of urokinase‑type plasminogen activator (uPA) and tissue‑type PA (1, 2). As important regulators of extracellular matrix remodeling, uPA and PAI‑1 play a major role in many processes such as angiogenesis, tumor invasion and obesity (2‑4). For example, uPA and PAI-1 are the only tumor prognostic factors validated at the highest level of evidence with regard to their clinical utility in breast cancer (5). The human PAI-1 is initially synthesized as 402 amino acid precursor with a N‑terminal signal peptide (6, 7). PAI‑1 may exist in one of two possible conformations, designated as active or latent (8). The purified rhPAI‑1 is active against rhuPA. The heterogeneity at the N‑terminus of the purified recombinant human PAI‑1 has been observed before for both the recombinant and native proteins (9). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010;56(0):1320.
Species: Human
Sample Type: Cell Culture Supernates
Application: Luminex Development - Human CD4- 8- T cells are a distinctive immunoregulatory subset.
Authors: Huang MC, Patel K, Taub DD, Longo DL, Goetzl EJ
FASEB J., 2010;24(7):2558-66.
Species: Human
Sample Type: Whole Cells
Application: Neut
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製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
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