抗Wnt-7α/β抗体(Anti-Wnt-7α/β, Goat-Poly antibody)
掲載日情報：2019/10/21 現在Webページ番号：31859
Wnt-7α/βに対する抗体(Anti-Wnt-7α/β, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月09日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Wnt-7α/β, Goat-Poly |
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Anti-Human Wnt-7a/b Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2024年05月09日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Wnt-7α/β, Goat-Poly
文献数： 4
- 商品コード：AF3008
- メーカー：RSD
- 包装：100μg
- 価格：¥100,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
別名：Wnt-7a Genbank No： 7476 Protein Accession No： O00755 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Goat | ||||
| 交差性 | Human | 適用 | IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
免疫染色システム ImmPRESS® Reagent Anti-Goat IgG Wnt関連製品 |
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| 関連記事 | |||||||
Anti-Human Wnt-7a/b Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF3008-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 Genbank No： 7476 Protein Accession No： O00755 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Human | 適用 | IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
|
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| 製品記事 |
免疫染色システム ImmPRESS® Reagent Anti-Goat IgG Wnt関連製品 |
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| 関連記事 | |||||||
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human Wnt‑7aLeu32-Glu72, Thr216-Arg292Accession # O00755 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human Wnt‑7a and Wnt-7b in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rmWnt-5b, rmWnt-9a, rmWnt-11, and rmWnt‑16 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human Wnt‑7a (Catalog # 3008-WN) and Recombinat Human Wnt-7b |
| Immunohistochemistry | 5-15 µg/mL | See below |
| Immunohistochemistry | |
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 | Wnt‑7a/b in Human endometrial Cancer Tissue. Wnt‑7a/b was detected in immersion fixed paraffin-embedded sections of human endometrial cancer tissue using 15 µg/mL Goat Anti-HumanWnt‑7a/b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3008) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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Related Product & Information
| Background | Wnt-7a/b |
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| background_content | Background: Wnt-7a/b Human Wnt-7a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to receptors of the Frizzled family in conjunction with low-density lipoprotein receptor‑related proteins (LRPs). Downstream effects of Wnt signaling occur through activation of one of three different intracellular pathways: the canonical Wnt pathway, Wnt/Ca2+ pathway, and planar cell polarity. The highly transforming Wnts, including Wnt-1, -3, -3a, -7a and -8 activate the canonical pathway, which regulates beta ‑catenin-mediated gene expression (1, 2). Human Wnt‑7a is a 48 kDa secreted glycoprotein containing 24 cysteine residues that is expressed by epithelial and epithelially-derived cells of the placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain (3, 4). Palmitate modification of a cysteine residue has been shown for Wnt‑3a; this site is conserved on all Wnts and is Cys73 on Wnt‑7a (5). When modified, increased hydrophobicity and activity is expected. Human Wnt‑7a shows 97% aa identity with mouse, rat, and dog Wnt‑7a and 92% aa identity with chicken Wnt‑7a. During development, Wnt‑7a is expressed by the dorsal ectoderm and drives expression of homeodomain transcription factors that control effectors important in patterning and cell fates in adjacent mesenchyme (6-10). When Wnt‑7a is deleted, mice show disruption of dorsalization and anterior/posterior patterning during limb development and abnormalities in the reproductive tract (6-10). Wnt‑7a is frequently downregulated in leukemia and lung cancers, potentially affecting homeobox (HOX) gene expression, differentiation state and growth control (11, 12). Roles for Wnt‑7a have also been shown during formation of neural synapses, response of the uterus to estrogen and inflammatory cartilage destruction (10, 13, 14). Wnt-7b is a 46 kDa, secreted glycosylated protein that belongs to the Wnt family. Wnt proteins can be lipid-modified and are ligands for members of the frizzled family of receptors, which mediates cell-cell communication during development. Human Wnt-7b is synthesized as a 349 amino acid (aa) precursor that contains a 318 aa mature region. The mature region contains 24 cysteines and three potential N-linked glycosylation sites. Wnt-7b is a ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Mature human Wnt-7b shares 99% aa sequence identity with mature mouse and rat Wnt-7b. Wnt-7b also shares 80% aa sequence identity with Wnt-7a. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation
Authors: Bruce F Kimler
J. Endocrinol., 2016;229(2):197-207.
Species: Rat
Sample Type: Whole Tissue
Application: IHC-P - CD82 inhibits canonical Wnt signalling by controlling the cellular distribution of beta-catenin in carcinoma cells.
Authors: Chigita S, Sugiura T, Abe M, Kobayashi Y, Shimoda M, Onoda M, Shirasuna K
Int J Oncol, 2012;41(6):2021-8.
Species: Human
Sample Type: Cell Lysates
Application: WB - Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus.
Authors: Gogolla N, Galimberti I, Deguchi Y, Caroni P
Neuron, 2009;62(4):510-25.
Species: Mouse
Sample Type: Tissue Homogenates
Application: WB
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