抗MMR抗体(Anti-MMR, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206Leu19-Ala1388Accession # Q2HZ94
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs, less than 45% cross-reactivity with recombinant human MMR is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	J774 mouse monocyte/macrophage cell line
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot
	Detection of Mouse MMR/CD206 by Western Blot. Western blot shows lysates of mouse liver tissue. Gels were loaded with 12 µg, 6.5 µg, and 3 µg of tissue lysate. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMR/CD206 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	MMR/CD206 in Mouse Testis. MMR/CD206 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spermatocytes in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
	MMR/CD206 in Mouse Lung. MMR/CD206 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of macrophages. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

MMR/CD206

background_content

Background: MMR/CD206 The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). Mouse to human, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (6). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor recycles to the cell surface (3, 8). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

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Citations

1. Salidroside provides neuroprotection by modulating microglial polarization after cerebral ischemia
   Authors: X Liu, S Wen, F Yan, K Liu, L Liu, L Wang, S Zhao, X Ji
   J Neuroinflammation, 2018;15(1):39.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


2. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
   Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
   J. Immunol, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Predictive screening of M1 and M2 macrophages reveals the immunomodulatory effectiveness of post spinal cord injury azithromycin treatment
   Authors: JC Gensel, TJ Kopper, B Zhang, MB Orr, WM Bailey
   Sci Rep, 2017;7(0):40144.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


4. ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury
   Authors: Y Yang, H Liu, H Zhang, Q Ye, J Wang, B Yang, L Mao, W Zhu, RK Leak, B Xiao, B Lu, J Chen, X Hu
   J. Neurosci., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. HDAC3 inhibition ameliorates spinal cord injury by immunomodulation
   Authors: T Kuboyama, S Wahane, Y Huang, X Zhou, JK Wong, A Koemeter-C, M Martini, RH Friedel, H Zou
   Sci Rep, 2017;7(1):8641.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC OCT-embedded


6. Targeting mannose receptor expression on macrophages in atherosclerotic plaques of apolipoprotein E-knockout mice using (111)In-tilmanocept
   Authors: Z Varasteh, F Hyafil, N Anizan, D Diallo, R Aid-Launai, S Mohanta, Y Li, M Braeuer, K Steiger, J Vigne, Z Qin, SG Nekolla, JE Fabre, Y Döring, D Le Guludec, A Habenicht, DR Vera, M Schwaiger
   EJNMMI Res, 2017;7(1):40.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3?
   Sci Rep, 2016;6(0):36698.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. miR-155 Deletion in Mice Overcomes Neuron-Intrinsic and Neuron-Extrinsic Barriers to Spinal Cord Repair
   J Neurosci, 2016;36(32):8516-32.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


9. Temporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury
   Front Cell Neurosci, 2016;10(0):286.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


10. Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
    Authors: Kan M, Lee J, Wilson J, Everhart A, Brown C, Hoofnagle A, Jansen M, Vitek M, Gunn M, Colton C
    J Neurosci, 2015;35(15):5969-82.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen

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