抗Human VEGF 165b抗体(Anti-Human VEGF 165b antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	KLH-conjugated human VEGF165b synthetic peptideTCRSLTRKDAccession # AAL27435
Source	Monoclonal Mouse IgG1 Clone # 56-1
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human VEGF165b in direct ELISAs and Western blots. Recognizes an epitope within a 9 amino acid sequence at the C-terminus of human VEGF165b. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) VEGF206, rhVEGF-B167, rhVEGF-B186, rhVEGF-C, or rhVEGF-D is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human VEGF165b (Catalog # 3045-VE)
Immunohistochemistry	Bates, D.O. et al. (2006) Clinical Sci. 110:575.
Immunofluorescence	Woolard, J. et al. (2004) Canc. Res. 64:7822.

Immunohistochemistry

VEGF165b in Human Placenta. VEGF165b was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human VEGF165b Monoclonal Antibody (Catalog # MAB3045) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Long Name	Vascular Endothelial Growth Factor
Background	VEGF
background_content	Background: VEGF VEGF165b is the protein resulting from an mRNA splice variant of VEGF. Alternative exon usage results in a 165 amino acid product that is identical to VEGF165 except for 6 unique amino acids at the C-terminus. VEGF165b inhibits proliferation and migration of endothelial cells and is down-regulated in renal cell carcinomas.

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Citations

1. Bone marrow-derived cells are implicated as a source of lymphatic endothelial progenitors in human breast cancer.
   Authors: Van't Hull E, Bron S, Henry L, Ifticene-Treboux A, Turrini R, Coukos G, Delaloye J, Doucey M
   Oncoimmunology, 2014;3(0):e29080.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. The role of VEGF-A165b in trophoblast survival.
   Authors: Bills V, Hamdollah-Zadeh M, Soothill P, Harper S, Bates D
   BMC Pregnancy Childbirth, 2014;14(0):278.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Detection of VEGF-A(xxx)b isoforms in human tissues.
   Authors: Bates, David O, Mavrou, Athina, Qiu, Yan, Carter, James G, Hamdollah-Zadeh, Maryam, Barratt, Shaney, Gammons, Melissa, Millar, Ann B, Salmon, Andrew H, Oltean, Sebastia, Harper, Steven J
   PLoS ONE, 2013;8(7):e68399.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. The transcription factor E2F1 and the SR protein SC35 control the ratio of pro-angiogenic versus antiangiogenic isoforms of vascular endothelial growth factor-A to inhibit neovascularization in vivo.
   Authors: Merdzhanova G, Gout S, Keramidas M, Edmond V, Coll JL, Brambilla C, Brambilla E, Gazzeri S, Eymin B
   Oncogene, 2010;29(39):5392-403.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


5. VEGF(121)b, a new member of the VEGF(xxx)b family of VEGF-A splice isoforms, inhibits neovascularisation and tumour growth in vivo.
   Authors: Rennel ES, Varey AH, Churchill AJ, Wheatley ER, Stewart L, Mather S, Bates DO, Harper SJ
   Br. J. Cancer, 2009;101(7):1183-93.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term.
   Authors: Bates DO, MacMillan PP, Manjaly JG, Qiu Y, Hudson SJ, Bevan HS, Hunter AJ, Soothill PW, Read M, Donaldson LF, Harper SJ
   Clin. Sci., 2006;110(5):575-85.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: ELISA Development


7. VEGF in the lung: a role for novel isoforms.
   Authors: Varet, Julia, Varet J, Douglas SK, Douglas, Samantha, Gilmartin, Laura, Gilmartin L, Medford, Andrew R, Bates, David O, Harper, Steven J, Millar, Ann B
   Am J Physiol Lung Cell Mol Physiol, 0;298(6):L768-74.
   Species: Human
   Sample Type: BALF
   Application: ELISA Development

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