抗Human Cripto-1抗体(Anti-Human Cripto-1 antibody)
掲載日情報:2021/01/28 現在Webページ番号:31488
Human Cripto-1に対する抗体(Anti-Human Cripto-1 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 :2024年05月17日 00時01分現在]
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Anti-Human Cripto-1 MAb (Clone 89633) |
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Anti-Human Cripto-1 MAb (Clone 89633) |
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Anti-Human Cripto-1 MAb (Clone 89633) |
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[在庫・価格 :2024年05月17日 00時01分現在]
Anti-Human Cripto-1 MAb (Clone 89633)
文献数: 0
- 商品コード:MAB2772-100
- メーカー:RSD
- 包装:100μg
- 価格:¥97,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
別名:CFC-2 クローン:89633 Genbank No: 6997 Protein Accession No: P13385 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | 法規備考 | ||||||
抗原種 | 免疫動物 | Mouse | |||||
交差性 | Human | 適用 | Blocking,ELISA,FCM,IC | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | |||||||
関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Human Cripto-1 MAb (Clone 89633)
文献数: 0
- 商品コード:MAB2772-500
- メーカー:RSD
- 包装:500μg
- 価格:¥232,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
マッチドペア:Human/Primate Cripto-1 サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAM2773,スタンダードとして#145-CR-010を用いる。 別名:CFC-2 クローン:89633 Genbank No: 6997 Protein Accession No: P13385 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Mouse | |||||
交差性 | Human | 適用 | Blocking,ELISA,FCM,IC | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 |
抗幹細胞マーカー抗体/抗胚性幹細胞マーカー抗体 |
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関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Human Cripto-1 MAb (Clone 89633)
文献数: 0
- 商品コード:MAB2772-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
※受注発注品。形状:溶液または凍結乾燥 別名:CFC-2 クローン:89633 Genbank No: 6997 Protein Accession No: P13385 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Mouse | |||||
交差性 | Human | 適用 | Blocking,ELISA,FCM,IC | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | |||||||
関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Human, Primate |
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Label | Unconjugated |
Immunogen | E. coli-derived recombinant human Cripto-1Arg38-Tyr188Accession # P13385 |
Source | Monoclonal Mouse IgG1 Clone # 89633 |
Purification | Protein A or G purified from hybridoma culture supernatant |
Specificity | Detects human and primate Cripto-1 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant mouse Cripto-1, recombinant human (rh) EGF, rhTGF‑ alpha, rhTGF-beta 1, rhTGF-beta 2, rhTGF-beta 3, or rhCryptic is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Blockade of Receptor-ligand Interaction | In a functional ELISA, 0.1-0.5 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of Recombinant Human Cripto-1 (Catalog # 145-CR) to immobilized Recombinant Human Activin RIB/ALK-4 Fc Chimera (Catalog # 808-AR) coated at 2 µg/mL (100 µL/well). At 20 µg/mL, this antibody will block >90% of the binding. | |
Immunocytochemistry | 5-25 µg/mL | See below |
Human/Primate Cripto-1 Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human/Primate Cripto-1 Antibody (Catalog #MAB2772 ) |
ELISA Detection (Matched Antibody Pair) | 0.5-2.0 µg/mL | Human/Primate Cripto-1 Biotinylated Antibody (Catalog #BAM2773 ) |
ELISA Standard | Recombinant Human Cripto-1 Protein (Catalog #145-CR ) | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. |
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Data Examples
Immunocytochemistry |
click image to view larger | Cripto-1 in Human iPSCs. Cripto-1 was detected in immersion fixed human induced pluripotent stem cells (iPSCs) using Mouse Anti-Human/Primate Cripto-1 Monoclonal Antibody (Catalog # MAB2772) at 20 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips. |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: Cripto | Cripto is the founding member of the epidermal growth factor-CriptoFRL1Cryptic (EGF-CFC) family of signaling proteins that function in cancer and various developmental processes. These developmental processes include: formation of the germ layers and dorsal organizer, specification of anterior-posterior and left-right axes, and differentiation of heart muscle (1, 2). Other members of the EGF-CFC family include Cryptic, Xenopus FRL-1 and zebrafish OEP (one-eyed pinhead). Overall sequence identity between members of the family is low, but they do share several common domains: a variant EGF-like motif, a novel conserved |
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References
Rosa, F.M. (2002) Science’s STKE https://stke.sciencemag.org/. | |
Shen, M. and A. Schier (2000) Trends Genet. 16:303. | |
Yan, Y-T. et al. (2002) Mol. Cell Biol. 22:4439. | |
Salomon, D. et al. (2000) Endocrine-Rel. Cancer 7:199. | |
Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5193. | |
Cheng, S. et al. (2003) Genes & Dev. 17:31. | |
Bianco, C. et al. (2003) Cancer Research 63:1192. | |
Long Name: | Teratocarcinoma-derived Growth Factor/Cripto Growth Factor |
Entrez Gene IDs: | 6997 (Human); 21667 (Mouse) |
Alternate Names: | CFC-2; CR; Cripto; Cripto-1 growth factor; Cripto-1; CRIPTOCRGF; Epidermal growth factor-like cripto protein CR1; TDGF1; TDGF-1; teratocarcinoma-derived growth factor 1 |
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Related Research Areas
Cardiac Stem Cell Markers |
Embryonic and Induced Pluripotent Stem Cell Markers |
TGF-beta Family |
TGF-beta Superfamily |
TGF-beta Superfamily Modulators |
Wnt Receptors |
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Related Product & Information
Long Name | Teratocarcinoma-derived Growth Factor/Cripto Growth Factor |
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Entrez Gene IDs | 6997 (Human); 21667 (Mouse) |
Background | Cripto |
background_content | Background: Cripto Cripto is the founding member of the epidermal growth factor-CriptoFRL1Cryptic (EGF-CFC) family of signaling proteins that function in cancer and various developmental processes. These developmental processes include: formation of the germ layers and dorsal organizer, specification of anterior-posterior and left-right axes, and differentiation of heart muscle (1, 2). Other members of the EGF-CFC family include Cryptic, Xenopus FRL-1 and zebrafish OEP (one-eyed pinhead). Overall sequence identity between members of the family is low, but they do share several common domains: a variant EGF-like motif, a novel conservedcysteine-rich domain (called CFC domain), and a C-terminal hydrophobic region. Most EGF-CFC members have a glycosyl-phosphatidylinositol (GPI) anchoring site at the C-terminus and exist as extracellular membrane-anchored proteins. However, naturally-occurring soluble isoforms also exist. Human Cripto shares 66% and 28% amino acid identity with mouse Cripto and zebrafish OEP, respectively (2). Despite weak conservation in amino acid identity, EGF-CFC family members appear to function similarly in assays for phenotypic rescue of zebrafish oep mutants (2). Both secreted and membrane bound forms of Cripto demonstrate biological activity (3). Cripto, also known as CFC-2 or TDGF-1 (teratocarcinoma-derived growth factor), was originally isolated from an undifferentiated human teratocarcinoma cell line as a potential oncogene. It is overexpressed in many types of cancers and acts as a growth factor for tumors (4). Genetic evidence from mice and zebrafish points to a role for Cripto as an essential cofactor in Nodal signaling. Cripto and OEP mutants display defects in mesoderm induction and heart morphogenesis, similar to phenotypes seen in Nodal mutants (2).Cripto acts as a cofactor for Nodal by recruiting the Activin type I Receptor, ALK-4, leading to anAct RIIB-ALK4-Cripto-Nodal complex for signaling (1, 3). Cripto also forms a complex with activin and Act RIIs to block activin signaling (5). Studies have shown that other TGF-beta superfamily members such as Vg1 and GDF-1 also require EGF-CFC cofactors (6). Cripto can also activate mitogen-activated protein kinase (MAPK) and Akt pathways independently of Nodal by directly binding to a membrane-associated heparan sulfate proteoglycan, glypican-1 (7). |
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