抗Nestin抗体(Anti-Nestin, Rat, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant rat NestinMet544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)Accession # EDM00749
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects rat Nestin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Rat Nestin
Immunohistochemistry	5-15 µg/mL	Immersion fixed paraffin-embedded sections of rat embyo (E15)
Immunocytochemistry	5-15 µg/mL	See below

Immunocytochemistry
	Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed rat cortical stem cells using 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	beta ‑III Tubulin and Nestin in Rat Cortical Stem Cells. beta ‑III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse beta ‑III Tubulin Monoclonal (clone TuJ‑1) Antibody (Catalog # MAB1195) and 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta‑III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed rat cortical stem cells using Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

Nestin

background_content

Background: Nestin Nestin is a 240 kDa class VI intermediate filament protein that was first identified in neuroepithelial stem cells (1, 2). It has been used extensively as a marker to identify central nervous system (CNS) stem cell populations. In addition, Nestin has been found to be a marker for multi-lineage progenitor cells. Over the region used for immunization, rat Nestin shares 76% amino acid sequence homology with the mouse protein.

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Citations

1. Nestin-positive microglia in adult rat cerebral cortex.
   Authors: Takamori Y, Mori T, Wakabayashi T, Nagasaka Y, Matsuzaki T, Yamada H
   Brain Res., 2009;1270(0):10-8.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC Frozen


2. Pten deletion in adult neural stem/progenitor cells enhances constitutive neurogenesis.
   Authors: Gregorian C, Nakashima J, Le Belle J, Ohab J, Kim R, Liu A, Smith KB, Groszer M, Garcia AD, Sofroniew MV, Carmichael ST, Kornblum HI, Liu X, Wu H
   J. Neurosci., 2009;29(6):1874-86.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


3. Mechanisms of murine lacrimal gland repair after experimentally induced inflammation.
   Authors: Zoukhri D, Fix A, Alroy J, Kublin CL
   Invest. Ophthalmol. Vis. Sci., 2008;49(10):4399-406.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


4. Vasopressin mediates mitogenic responses to adrenalectomy in the rat anterior pituitary.
   Authors: Subburaju S, Aguilera G
   Endocrinology, 2007;148(7):3102-10.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC Frozen


5. Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
   Authors: De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD
   Biotechnol. Bioeng., 2007;97(6):1617-25.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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