抗Mouse IL-23 R抗体(Anti-Mouse IL-23 R antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse IL-23 RGly24-Asp372Accession # Q8K4B4
Source	Monoclonal Rat IgG2B Clone # 258010
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IL-23 R in Western blots. In Western blots, this antibody does not cross-react with recombinant human IL-23 R.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse IL‑23 R Fc Chimera (Catalog # 1686-MR)
Neutralization	Measured by its ability to neutralize IL‑23-induced IL‑17 secretion in mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 0.75 ng/mL Recombinant Mouse IL‑23 and 10 ng/mL Recombinant Mouse IL‑2.

Neutralization

IL‑17 Secretion Induced byIL‑23 and Neutralization by Mouse IL‑23 R Antibody. In the presence of Recombinant Mouse IL‑2 (10 ng/mL, Catalog # 402-ML), Recombinant Mouse IL‑23 (Catalog # 1887-ML) stimulates IL‑17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL‑17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL‑17 secretion elicited by Recombinant Mouse IL‑23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑23 R Monoclonal Antibody (Catalog # MAB1686). The ND50 is typically 0.1-0.4 µg/mL.

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Related Product & Information

Long Name	Interleukin 23 Receptor
Entrez Gene IDs	149233 (Human); 209590 (Mouse)
Background	IL-23 R
background_content	Background: IL-23 R Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4+ CD45RBlow memory T cells but at much lower levels by mouse CD4+ CD45RBhigh cells. IL-23 initiates a signal transduction cascade similar to that of IL-12 and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.

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Citations

1. Integration of T Cell Receptor, Notch and Cytokine Signals Programs in Mouse ?? T Cell Effector Differentiation
   Authors: P Zarin, TSH In, ELY Chen, J Singh, GW Wong, M Mohtashami, DL Wiest, MK Anderson, JC Zúñiga-Pfl
   Immunol. Cell Biol., 2018;0(0):.
   Species: Mouse
   Sample Type: Complex Sample Type
   Application: Neut


2. IL-23 Inhibits Melanoma Development by Augmenting DNA Repair and Modulating T Cell Subpopulations
   Authors: Tahseen H Nasti
   J. Immunol, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Staphylococcus aureus infection of mice expands a population of memory gammadelta T cells that are protective against subsequent infection.
   Authors: Murphy A, O'Keeffe K, Lalor S, Maher B, Mills K, McLoughlin R
   J Immunol, 2014;192(8):3697-708.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


4. IL-22 from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection.
   Authors: Kumar P, Thakar M, Ouyang W, Malarkannan S
   Mucosal Immunol, 2013;6(1):69-82.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
   Authors: Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L
   J. Exp. Med., 2011;208(3):491-503.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


6. A human colonic commensal promotes colon tumorigenesis via activation of T helper type 17 T cell responses.
   Authors: Wu S, Rhee KJ, Albesiano E, Rabizadeh S, Wu X, Yen HR, Huso DL, Brancati FL, Wick E, McAllister F, Housseau F, Pardoll DM, Sears CL
   Nat. Med., 2009;15(9):1016-22.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


7. Resident Vdelta1+ gammadelta T cells control early infiltration of neutrophils after Escherichia coli infection via IL-17 production.
   Authors: Shibata K, Yamada H, Hara H, Kishihara K, Yoshikai Y
   J. Immunol., 2007;178(7):4466-72.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


8. Cutting edge: An in vivo requirement for STAT3 signaling in TH17 development and TH17-dependent autoimmunity.
   Authors: Harris TJ, Grosso JF, Yen HR, Xin H, Kortylewski M, Albesiano E, Hipkiss EL, Getnet D, Goldberg MV, Maris CH, Housseau F, Yu H, Pardoll DM, Drake CG
   J. Immunol., 2007;179(7):4313-7.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo

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