抗Human/Mouse Oct-3/4 Phycoerythrin抗体(Anti-Human/Mouse Oct-3/4 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Phycoerythrin
Immunogen	E. coli-derived recombinant human Oct‑3/4Met1-Asn265 (Met262Leu)Accession # Q01860
Source	Monoclonal Rat IgG2B Clone # 240408
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry
	Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct‑3/4 PE‑conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
	Detection of Oct‑3/4 in Mouse Stem Cells by Flow Cytometry. Mouse embryonic stem cells treated with 5 μM Retinoic Acid for 3 days were stained with Rat Anti-Human/Mouse Oct‑3/4 PE‑conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background	Oct-3/4
background_content	Background: Oct-3/4 Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells (1- 3). Oct-3/4 is required to sustain stem cell self-renewal and pluripotency (4). It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

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Citations

1. Generation of hiPSTZ16 (ISMMSi003-A) cell line from normal human foreskin fibroblasts
   Authors: M Dejosez, TP Zwaka
   Stem Cell Res, 2017;26(0):44-46.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells
   PLoS ONE, 2016;11(9):e0161229.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Phenotypic diversity of patient-derived melanoma populations in stem cell medium.
   Authors: Sztiller-Sikorska M, Hartman M, Talar B, Jakubowska J, Zalesna I, Czyz M
   Lab Invest, 2015;95(6):672-83.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells.
   Authors: Huijbers I, Bin Ali R, Pritchard C, Cozijnsen M, Kwon M, Proost N, Song J, de Vries H, Badhai J, Sutherland K, Krimpenfort P, Michalak E, Jonkers J, Berns A
   EMBO Mol Med, 2014;6(2):212-25.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Scalable passaging of adherent human pluripotent stem cells.
   Authors: Nie Y, Walsh P, Clarke D, Rowley J, Fellner T
   PLoS ONE, 2014;9(1):e88012.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
   Gene Ther, 2012;19(10):1035-40.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Human embryonic stem cells with maintenance under a feeder-free and recombinant cytokine-free condition.
   Authors: Nakahara M, Saeki K, Nakamura N
   Cloning Stem Cells, 2009;11(1):5-18.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Octamer 4 small interfering RNA results in cancer stem cell-like cell apoptosis.
   Authors: Hu T, Liu S, Breiter DR, Wang F, Tang Y, Sun S
   Cancer Res., 2008;68(16):6533-40.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. Kidney tissue reconstruction by fetal kidney cell transplantation: effect of gestation stage of fetal kidney cells.
   Authors: Kim SS, Gwak SJ, Han J, Park HJ, Park MH, Song KW, Cho SW, Rhee YH, Chung HM, Kim BS
   Stem Cells, 2007;25(6):1393-401.
   Species: Rat
   Sample Type: Whole Cells
   Application: Flow

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