抗IL-2 Receptor β抗体(Anti-IL-2 Receptor β, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑2 R beta Ala27-Asp239Accession # NP_000869
Source	Monoclonal Mouse IgG1 Clone # 27302
Purification	Protein A or G purified from ascites
Specificity	Detects human IL-2 R beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R alpha, rh gamma c, rhIL‑4 R, or rhIL‑6 R is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of IL‑2 R beta in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human IL‑2 R beta PE‑conjugated Monoclonal Antibody (Catalog # FAB224P) and Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	Interleukin 2 Receptor beta
Background	IL-2 R beta
background_content	Background: IL-2 R beta Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. Individual beta chains and alpha chains exhibit low affinity IL-2 binding, while the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling. IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid (aa) precursor Type I membrane protein with a 26 aa signal peptide, a 214 aa extracellular region, a 25 aa transmembrane region and a 286 aa cytoplasmic domain. A soluble IL-2 R beta has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 R beta. Soluble IL-2 R beta binds IL-2 with low affinity and is not an effective IL-2 antagonist on cells displaying the high or intermediate affinity IL-2 signaling receptors. Nevertheless, soluble IL-2 R beta binds IL-15 with sufficient affinity to neutralize IL-15 biological activities.

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Citations

1. Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration.
   Authors: Arneja A, Johnson H, Gabrovsek L, Lauffenburger D, White F
   J Immunol, 2014;192(1):123-35.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Identification of human NK17/NK1 cells.
   Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
   PLoS ONE, 2011;6(10):e26780.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. A novel immunomodulatory mechanism of ribavirin in suppressing natural killer cell function.
   Authors: Ogbomo H, Michaelis M, Altenbrandt B, Doerr HW, Cinatl J
   Biochem. Pharmacol., 2010;79(2):188-97.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.
   Authors: Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH
   J. Immunol., 2008;180(11):7221-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Histone deacetylase inhibitors suppress natural killer cell cytolytic activity.
   Authors: Ogbomo H, Michaelis M, Kreuter J, Doerr HW, Cinatl J
   FEBS Lett., 2007;581(7):1317-22.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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