抗Nectin-4抗体(Anti-Nectin-4, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Nectin-4Gly27-Val351Accession # Q96NY8
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross‑reactivity with recombinant mouse Nectin-4 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human Nectin‑4 (Catalog # 2659-N4)
Flow Cytometry	0.25 µg/106 cells	MCF‑7 human breast cancer cell line
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

Nectin‑4 in Human Placenta. Nectin‑4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 µg/mL Goat Anti-Human Nectin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2659) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Long Name	Poliovirus Receptor Related 4
Background	Nectin-4
background_content	Background: Nectin-4 Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.

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Citations

1. Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs
   Authors: W Pratakpiri, AP Ping Teh, A Radtanakat, N Pirarat, N Thi Lan, M Takeda, S Techangams, R Yamaguchi
   Sci Rep, 2017;7(1):349.
   Species: Canine
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


2. A Novel Nectin-mediated Cell Adhesion Apparatus That Is Implicated in Prolactin Receptor Signaling for Mammary Gland Development
   Authors: M Kitayama, K Mizutani, M Maruoka, K Mandai, S Sakakibara, Y Ueda, T Komori, Y Shimono, Y Takai
   J. Biol. Chem, 2016;291(11):5817-31.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


3. Oncolytic measles virus expressing the sodium iodide symporter to treat drug-resistant ovarian cancer.
   Authors: Galanis E, Atherton P, Maurer M, Knutson K, Dowdy S, Cliby W, Haluska P, Long H, Oberg A, Aderca I, Block M, Bakkum-Gamez J, Federspiel M, Russell S, Kalli K, Keeney G, Peng K, Hartmann L
   Cancer Res, 2015;75(1):22-30.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Not specified


4. SLAM- and Nectin-4-Independent Noncytolytic Spread of Canine Distemper Virus in Astrocytes.
   Authors: Alves L, Khosravi M, Avila M, Ader-Ebert N, Bringolf F, Zurbriggen A, Vandevelde M, Plattet P
   J Virol, 2015;89(10):5724-33.
   Species: Canine
   Sample Type: Whole Tissue
   Application: IHC


5. Nectin-4 expression contributes to tumor proliferation, angiogenesis and patient prognosis in human pancreatic cancer.
   Authors: Nishiwada S, Sho M, Yasuda S, Shimada K, Yamato I, Akahori T, Kinoshita S, Nagai M, Konishi N, Nakajima Y
   J Exp Clin Cancer Res, 2015;34(0):30.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - paraffin


6. Nectin-4 mutations causing ectodermal dysplasia with syndactyly perturb the rac1 pathway and the kinetics of adherens junction formation.
   Authors: Fortugno P, Josselin E, Tsiakas K, Agolini E, Cestra G, Teson M, Santer R, Castiglia D, Novelli G, Dallapiccola B, Kurth I, Lopez M, Zambruno G, Brancati F
   J Invest Dermatol, 2014;134(8):2146-53.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. Lethal canine distemper virus outbreak in cynomolgus monkeys in Japan in 2008.
   J Virol, 2013;87(2):1105-14.
   Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. A role for PVRL4-driven cell-cell interactions in tumorigenesis.
   Authors: Pavlova N, Pallasch C, Elia A, Braun C, Westbrook T, Hemann M, Elledge S
   Elife, 2013;2(0):e00358.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


9. Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus.
   Authors: Noyce RS, Bondre DG, Ha MN
   PLoS Pathog., 2011;7(8):e1002240.
   Species: Hamster
   Sample Type: Whole Cells
   Application: Neut

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