抗Musashi-1抗体(Anti-Musashi-1, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human Musashi-1Met1-His362Accession # O43347
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Musashi-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Musashi‑2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Immunohistochemistry	1-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Recombinant Human Musashi‑1 by Western Blot. Western blot shows 25 ng of Recombinant Human Musashi-1 and Recombinant Human Musashi-2. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Musashi‑1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunocytochemistry
	Musashi‑1 in Mouse Cortical Stem Cells. Musashi‑1 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human/Mouse/Rat Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Immunocytochemistry
	Musashi‑1 in Rat Cortical Stem Cells. Musashi‑1 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human/Mouse/Rat Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Immunohistochemistry
	Musashi‑1 in Human Intestine. Musashi‑1 was detected in immersion fixed paraffin-embedded sections of human intestine using 1 µg/mL Goat Anti-Human/Mouse/Rat Musashi‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Background	Musashi-1
background_content	Background: Musashi-1 Musashi-1 is an RNA-binding protein that is expressed in the developing central nervous system. Expression is highest in proliferating multipotent neural precursor cells and decreases during neuronal differentiation (1, 2).

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Citations

1. Rapid generation of OPC-like cells from human pluripotent stem cells for treating spinal cord injury
   Authors: DS Kim, SJ Jung, JS Lee, BY Lim, HA Kim, JE Yoo, DW Kim, JW Leem
   Exp. Mol. Med., 2017;49(7):e361.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Musashi-1 is the candidate of the regulator of hair cell progenitors during inner ear regeneration
   Authors: T Wakasaki, H Niiro, S Jabbarzade, M Ohashi, T Kimitsuki, T Nakagawa, S Komune, K Akashi
   BMC Neurosci, 2017;18(1):64.
   Species: Chicken
   Sample Type: Whooe Tissue
   Application: IHC OCT-embedded


3. Expression and regulation of prostate apoptosis response-4 (Par-4) in human glioma stem cells in drug-induced apoptosis.
   Authors: Jagtap, Jayashre, Dawood, Parveen, Shah, Reecha D, Chandrika, Goparaju, Natesh, Kumar, Shiras, Anjali, Hegde, Amba S, Ranade, Deepak, Shastry, Padma
   PLoS ONE, 2014;9(2):e88505.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Side populations of gastrointestinal cancers are not enriched in stem cells.
   Authors: Burkert J, Otto WR, Wright NA
   J. Pathol., 2008;214(5):564-73.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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