抗Akt1抗体(Anti-Akt1, Pan, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human Akt1Ser2-Ala480Accession # P31749
Source	Monoclonal Mouse IgG2B Clone # 281046
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human, mouse and rat Akt in direct ELISAs and Western blots.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Simple Western	2 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	See below

Western Blot
	Detection of Human/Mouse Akt by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, MBA-MB-123 human breast cancer cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody (Catalog # MAB2055) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Western Blot
	Detection of Human Akt Pan Specific by Western Blot. Western blot shows Recombinant Human Active Akt1 (Catalog # 1775-KS), recombinant human Akt2, and recombinant human Akt3 (5 ng/lane). PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody (Catalog # MAB2055) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Immunocytochemistry
	Akt in MDA‑MB‑231 Human Cell Line. Akt was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat Akt Monoclonal Antibody (Catalog # MAB2055) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Intracellular Staining by Flow Cytometry
	Detection of Akt in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human/Mouse/Rat Akt Monoclonal Antibody (Catalog # MAB2055, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Immunocytochemistry
	Akt (pan) in 293T Human Cell Line. Akt pan specific (panels A and C) and Akt1 (E17K Mutation) (panels B and D) were detected in immersion fixed 293T human embryonic kidney cell line transfected with wild type (panels A and B) or E17K mutated (panels C and D) Akt1 using Mouse Anti-Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody (Catalog # MAB2055) and Mouse Anti-Human Akt1 (E17K Mutation) Monoclonal Antibody (Catalog # MAB6815). Both antibodies were used at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western
	Detection of Human Akt by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Akt at approximately 62 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody (Catalog # MAB2055). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

目次に戻る

Related Product & Information

Long Name	v-Akt Murine Thymoma Viral Oncogene Homolog
Background	Akt
background_content	Background: Akt Akt, also known as protein kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.

目次に戻る

Citations

1. Subthalamic Nucleus Deep Brain Stimulation Employs trkB Signaling for Neuroprotection and Functional Restoration
   Authors: DL Fischer, CJ Kemp, A Cole-Strau, NK Polinski, KL Paumier, JW Lipton, K Steece-Col, TJ Collier, DJ Buhlinger, CE Sortwell
   J. Neurosci., 2017;37(28):6786-6796.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Frozen


2. Adipocyte SIRT1 controls systemic insulin sensitivity by modulating macrophages in adipose tissue
   Authors: X Hui, M Zhang, P Gu, K Li, Y Gao, D Wu, Y Wang, A Xu
   EMBO Rep, 2017;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


3. Endocrine responses and acute mTOR pathway phosphorylation to resistance exercise with leucine and whey
   Authors: MT Lane, TJ Herda, AC Fry, MA Cooper, MJ Andre, PM Gallagher
   Biol Sport, 2017;34(2):197-203.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: Infared Microscopy


4. Lack of CD2AP disrupts Glut4 trafficking and attenuates glucose uptake in podocytes.
   Authors: Tolvanen T, Dash S, Polianskyte-Prause Z, Dumont V, Lehtonen S
   J Cell Sci, 2015;128(24):4588-600.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


5. Single amino Acid substitutions in the chemotactic sequence of urokinase receptor modulate cell migration and invasion.
   Authors: Bifulco, Katia, Longanesi-Cattani, Immacola, Franco, Paola, Pavone, Vincenzo, Mugione, Pietro, Di Carluccio, Gioconda, Masucci, Maria Te, Arra, Claudio, Pirozzi, Giuseppe, Stoppelli, Maria Pa, Carriero, Maria Vi
   PLoS ONE, 2012;7(9):e44806.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt.
   Authors: Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S
   Apoptosis, 2010;15(4):463-73.
   Species: Rat
   Sample Type: Cell Lysates
   Application: WB


7. Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation.
   Authors: Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C
   J. Mol. Cell. Cardiol., 2010;48(6):1225-35.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

目次に戻る