抗Integrin β1抗体(Anti-Integrin β1, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse, Rat
Label	Unconjugated
Immunogen	Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1Gln21-Ala738Accession # P09055
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse Integrin beta 1/CD29 in direct ELISAs. Detects mosue and rat Integrin beta 1/CD29 in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Integrin beta 1 and rhIntegrin beta 3 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin beta 2, rmIntegrin beta 4, and rmIntegrin beta 6 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Flow Cytometry	2.5 µg/106 cells	B16‑F10 mouse melanoma cell line
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot
	Detection of Mouse and Rat Integrin  beta 1/CD29 by Western Blot. Western blot shows lysates of L‑929 mouse fibroblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, PC‑3 human prostate cancer cell line, Rat‑2 rat embryonic fibroblast cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Integrin  beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2405) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin  beta 1/CD29 at approximately 110-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem. Orexin B, p38 alpha, and Integrin  beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Mouse/Rat Integrin  beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Simple Western
	Detection of Mouse and Rat Integrin  beta 1/CD29 by Simple WesternTM. Simple Western lane view shows lysates of L‑929 mouse fibroblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, PC‑12 rat adrenal pheochromocytoma cell line, and Rat‑2 rat embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin  beta 1/CD29 at approximately 153-174 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin  beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2405) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

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Related Product & Information

Entrez Gene IDs	3688 (Human); 16412 (Mouse)
Background	Integrin beta 1/CD29
background_content	Background: Integrin beta 1/CD29 The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.

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Citations

1. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
   Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
   Nat. Cell Biol, 2017;19(3):189-201.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Exon skipping restores dystrophin expression, but fails to prevent disease progression in later stage dystrophic dko mice.
   Authors: Wu, B, Cloer, C, Lu, P, Milazi, S, Shaban, M, Shah, S N, Marston-Poe, L, Moulton, H M, Lu, Q L
   Gene Ther, 2014;21(9):785-93.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Fresh


3. Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5.
   Authors: Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST
   J. Neurochem., 2011;118(4):521-32.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: IP


4. Loss of beta1 integrin in mouse fibroblasts results in resistance to skin scleroderma in a mouse model.
   Authors: Liu S, Kapoor M, Denton CP, Abraham DJ, Leask A
   Arthritis Rheum., 2009;60(9):2817-21.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


5. Galectin-7 in the control of epidermal homeostasis after injury.
   Authors: Gendronneau G, Sidhu SS, Delacour D, Dang T, Calonne C, Houzelstein D, Magnaldo T, Poirier F
   Mol. Biol. Cell, 2008;19(12):5541-9.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
   Authors: Chang Y, Finnemann SC
   J. Cell. Sci., 2007;120(0):3053-63.
   Species: Rat
   Sample Type: Cell Lysates
   Application: IP

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