抗HNF-3 β抗体(Anti-HNF-3 β, Human, Goat-Poly antibody)

掲載日情報:2018/11/26 現在Webページ番号:31096

HNF-3 βに対する抗体(Anti-HNF-3 β, Human, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

価格

[在庫・価格 :2024年04月24日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-HNF-3 β, Human, Goat-Poly <Anti-FoxA2>
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 50
説明文
別名:forkhead box A2
Genbank No: 3170
Protein Accession No: Q9Y261
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 ChIP,IC,IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 StemXVivo Endoderm / Ectoderm / Mesoderm Kit
免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
関連記事
Anti-Human HNF-3 beta/FoxA2 Affinity Purified Polyclonal Ab
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 16
説明文
※受注発注品。形状:溶液または凍結乾燥
別名:forkhead box A2
Genbank No: 3170
Protein Accession No: Q9Y261
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 ChIP,IC,IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
関連記事

[在庫・価格 :2024年04月24日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-HNF-3 β, Human, Goat-Poly <Anti-FoxA2>

文献数: 50

説明文 別名:forkhead box A2
Genbank No: 3170
Protein Accession No: Q9Y261
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat
交差性 Human 適用 ChIP,IC,IHC,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 StemXVivo Endoderm / Ectoderm / Mesoderm Kit
免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
関連記事

Anti-Human HNF-3 beta/FoxA2 Affinity Purified Polyclonal Ab

文献数: 16

説明文 ※受注発注品。形状:溶液または凍結乾燥
別名:forkhead box A2
Genbank No: 3170
Protein Accession No: Q9Y261
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat
交差性 Human 適用 ChIP,IC,IHC,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
関連記事



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Product Details

Species ReactivityHuman
LabelUnconjugated
ImmunogenE. coli-derived recombinant human HNF‑3 beta /FoxA2Met242-Ser457Accession # Q9Y261
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects human HNF‑3 beta /FoxA2 in direct ELISAs and Western blots.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLSee below
Chromatin Immunoprecipitation (ChIP)5 µg/5 x 106 cellsSee below
Immunocytochemistry5-15 µg/mLSee below


Western Blot
Detection of Human HNF‑3 beta /FoxA2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HNF‑3 beta /FoxA2 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chromatin Immunoprecipitation (ChIP)
Detection of HNF‑3 beta /FoxA2-regulated Genes by Chromatin Immunoprecipitation.
Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF‑3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The E-RABP promoter was detected by standard PCR.
Immunocytochemistry
HNF‑3 beta /FoxA2 in Endoderm Differentiated BG01V Human Stem Cells.
HNF‑3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.


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Related Product & Information

Long NameHepatocyte Nuclear Factor-3, beta
BackgroundHNF-3 beta/FoxA2
background_contentBackground:
HNF-3 beta/FoxA2
HNF-3 beta, also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells
    Authors: Y Kondo, T Toyoda, R Ito, M Funato, Y Hosokawa, S Matsui, T Sudo, M Nakamura, C Okada, X Zhuang, A Watanabe, A Ohta, N Inagaki, K Osafune
    Diabetologia, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

  2. Generation of thalamic neurons from mouse embryonic stem cells
    Authors: A Shiraishi, K Muguruma, Y Sasai
    Development, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC

  3. Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS)
    Authors: B Kuebler, B Aran, L Miquel-Ser, Y Muñoz, E Ars, G Bullich, M Furlano, R Torra, M Marti, A Veiga, A Raya
    Stem Cell Res, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

  4. Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines
    Authors: L Miquel-Ser, A Duarri, Y Muñoz, B Kuebler, B Aran, C Costa, M Martí, M Comabella, S Malhotra, X Montalban, A Veiga, A Raya
    Stem Cell Res, 2017;24(0):155-159.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

  5. CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells
    Authors: KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima
    Stem Cell Reports, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

  6. Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells
    Authors: F Sampazioti, MC de Brito, I Geti, A Bertero, NR Hannan, L Vallier
    Nat Protoc, 2017;12(4):814-827.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

  7. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
    Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
    Cell Rep, 2017;19(1):20-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

  8. Triple Staining Including FOXA2 Identifies Stem Cell Lineages Undergoing Hepatic and Biliary Differentiation in Cirrhotic Human Liver
    Authors: Leslie E Rogler
    J. Histochem. Cytochem., 2016;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: ICC

  9. Cell Expansion During Directed Differentiation Of Stem Cells Toward The Hepatic Lineage
    Stem Cells Dev., 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: CyTof

  10. Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells
    Sci Rep, 2016;6(0):31063.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC-Fluorescent



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