抗Lipocalin-2抗体(Anti-Lipocalin-2, Goat-Poly, Biotin antibody)
掲載日情報:2021/01/28 現在Webページ番号:30920
Lipocalin-2に対する抗体(Anti-Lipocalin-2, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 :2024年05月18日 00時00分現在]
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Anti-Lipocalin-2, Goat-Poly, Biotin <Anti-NGAL> |
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[在庫・価格 :2024年05月18日 00時00分現在]
Anti-Lipocalin-2, Goat-Poly, Biotin <Anti-NGAL>
文献数: 2
- 商品コード:BAF1757
- メーカー:RSD
- 包装:50μg
- 価格:¥119,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
マッチドペア:Human Lipocalin-2/NGAL サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#MAB17571-500,スタンダードとして#1757-LC-050を用いる。 別名:24p3 Genbank No: 3934 Protein Accession No: P80188 |
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法規制等 | |||
保存条件 | -20℃ | 法規備考 | |
抗原種 | Human | 免疫動物 | Goat |
交差性 | Human | 適用 | ELISA,Western Blot |
標識 | Biotin | 性状 | Antigen Affinity Purified |
吸収処理 | クラス | IgG | |
クロナリティ | Polyclonal | フォーマット | |
掲載カタログ |
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製品記事 | |||
関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Human |
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Label | Biotin |
Immunogen | Mouse myeloma cell line NS0-derived recombinant human Lipocalin-2/NGAL (R&D Systems, Catalog # 1757-LC)Gln21-Gly198Accession # Q6FGL5 |
Source | Polyclonal Goat IgG |
Purification | Antigen Affinity-purified |
Specificity | Detects human Lipocalin-2/NGAL in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human Lipocalin‑1, recombinant mouse Lipocalin-2, and recombinant rat Lipocalin-2 is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 0.1 µg/mL | Recombinant Human Lipocalin‑2/NGAL (Catalog # 1757-LC) |
Human Lipocalin-2/NGAL Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human Lipocalin‑2/NGAL Antibody (Catalog #MAB17571R ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human Lipocalin‑2/NGAL Biotinylated Antibody (Catalog #BAF1757 ) |
ELISA Standard | Recombinant Human Lipocalin-2/NGAL Protein, CF (Catalog #1757-LC ) | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: Lipocalin-2/NGAL | Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells (1). Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules (2). It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9) (2). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation (3). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3 - 5). Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM) (6). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8). |
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References
Flower, D.R. et al. (1994) FEBS Lett. 354:7. | |
Kjeldsen, L. et al. (1993) J. Biol. Chem. 268:10426. | |
Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272. | |
Devireddy, L.R. et al. (2001) Science 293: 829. | |
Yang, M.B. et al. (2002) Mol. Cell. 10:1045. | |
Goetz, D.H. et al. (2002) Mol. Cell 10:1033. | |
Flo, T.H. et al. (2004) Nature 432:917. | |
Hanai, J. et al. (2005) J. Biol. Chem. 280:13641. | |
Long Name: | Neutrophil Gelatinase-associated Lipocalin |
Entrez Gene IDs: | 3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey) |
Alternate Names: | 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin |
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Related Research Areas
Adipocytokines | ||
Cancer Biomarkers | ||
Metalloproteases and Regulators | ||
MMPs, TIMPs and Related Molecules | ||
Renal Markers | ||
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Related Product & Information
Background | Lipocalin-2/NGAL |
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background_content | Background: Lipocalin-2/NGAL Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells (1). Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules (2). It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9) (2). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation (3). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3 - 5). Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM) (6). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8). |
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Citations
- Pathogenic Upregulation of Glial Lipocalin-2 in the Parkinsonian Dopaminergic System
J Neurosci, 2016;36(20):5608-22.
Species: Human
Sample Type: Tissue Homogenates
Application: WB
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