抗Olig2抗体(Anti-Olig2, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human Olig2Met1-Lys323Accession # Q13516
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Olig2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Olig3 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human Olig2
Immunohistochemistry	5-15 µg/mL	See below
Chromatin Immunoprecipitation (ChIP)	5 µg/5 x 106 cells	See below
Immunocytochemistry	5-15 µg/mL	See below

Immunocytochemistry
	Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells. Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 µg/mL Goat Anti-Human/Mouse/Rat/Chicken O4 Monoclonal Antibody (Catalog # MAB1326). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Olig2 using the NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002), and stained for O4 using an anti-mouse IgM secondary antibody (pseudo-stained green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Chromatin Immunoprecipitation (ChIP)
	Detection of Olig2-regulated Genes by Chromatin Immunoprecipitation. A172 human glioblastoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Olig2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.
Immunohistochemistry
	Olig2 in Mouse Embryo. Olig2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Long Name	Oligodendrocyte Lineage Transcription Factor 2
Background	Olig2
background_content	Background: Olig2 Olig1 and Olig2 are basic-helix-loop-helix (bHLH) transcription factors expressed in the motor neuron progenitor (pMN) domain of the spinal cord that generates motor neurons and oligodendrocytes. Olig1 is involved in the development and maturation of oligodendrocytes. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord.

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Citations

1. A TLR/AKT/FoxO3 immune tolerance-like pathway disrupts the repair capacity of oligodendrocyte progenitors
   Authors: T Srivastava, P Diba, JM Dean, F Banine, D Shaver, M Hagen, X Gong, W Su, B Emery, DL Marks, EN Harris, B Baggenstos, PH Weigel, LS Sherman, SA Back
   J. Clin. Invest., 2018;0(0):.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


2. Targeting Glioma Stem Cell-Derived Pericytes Disrupts the Blood-Tumor Barrier and Improves Chemotherapeutic Efficacy
   Authors: W Zhou, C Chen, Y Shi, Q Wu, RC Gimple, X Fang, Z Huang, K Zhai, SQ Ke, YF Ping, H Feng, JN Rich, JS Yu, S Bao, XW Bian
   Cell Stem Cell, 2017;21(5):591-603.e4.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


3. The doublesex-related Dmrta2 safeguards neural progenitor maintenance involving transcriptional regulation of Hes1
   Authors: FI Young, M Keruzore, X Nan, N Gennet, EJ Bellefroid, M Li
   Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


4. Delayed treatment of secondary degeneration following acute optic nerve transection using a combination of ion channel inhibitors
   Authors: NJ Yates, MK Giacci, RL O'Hare Doi, W Chiha, BE Ashworth, J Kenna, CA Bartlett, M Fitzgerald
   Neural Regen Res, 2017;12(2):307-316.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC OCT-embedded


5. RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation
   Authors: Qi Xie
   J Clin Invest, 2016;126(7):2757-2772.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. The intellectual disability protein PAK3 regulates oligodendrocyte precursor cell differentiation
   Authors: Majistor Raj Luxman Maglo Renkilaraj
   Neurobiol. Dis, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


7. Brain tissue banking for stem cells for our future
   Sci Rep, 2016;6(0):39394.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: IHC Not Specified


8. A cord blood monocyte-derived cell therapy product accelerates brain remyelination
   JCI Insight, 2016;1(13):e86667.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Not Specified


9. Treatment Outcome and Prognostic Molecular Markers of Supratentorial Primitive Neuroectodermal Tumors
   Authors: SH Choi, SH Kim, KW Shim, JW Han, J Choi, DS Kim, CJ Lyu, JW Kim, CO Suh, J Cho
   PLoS ONE, 2016;11(4):e0153443.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


10. Olig2 is expressed late in human eosinophil development and controls Siglec-8 expression
    J Leukoc Biol, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: ChIP Assay

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