抗TIM-3抗体(Anti-TIM-3, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human TIM‑3Ser22-Arg200Accession # Q8TDQ0
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human TIM-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) TIM‑3 is observed and less than 1% cross-reactivity with recombinant human TIM-1, rmTIM-1, and rmTIM-2 is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human TIM‑3 Fc Chimera (Catalog # 2365-TM)
Flow Cytometry	2.5 µg/106 cells	Human peripheral blood monocytes
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

目次に戻る

Related Product & Information

Background

TIM-3

background_content

Background: TIM-3 TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). There are three TIM genes in human and eight in mouse. Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+ Tc1) but not on helper T cells (CD4+ Th2 and CD8+ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. It also occurs on hippocampal neurons (7-10). The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 11). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling (8, 12). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 10, 11, 13), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (14). Soluble mouse TIM-3 constructs which lack the cytoplasmic domain have been shown to inhibit anti-tumor effector T cell responses and to enhance autoimmune reactions (5, 15).

目次に戻る

Citations

1. Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9
   Authors: JA Schwartz, KL Clayton, S Mujib, H Zhang, AK Rahman, J Liu, FY Yue, E Benko, C Kovacs, MA Ostrowski
   J. Immunol, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Galectin-9 as a Predictive Marker for the Onset of Immune-Related Adverse Effects Associated with Anti-CCR4 MoAb Therapy in Patients with Adult T Cell Leukemia
   Authors: TO Mohammed, H Chagan-Yas, Y Ashino, W Nakayama, Y Takahashi, T Shimomura, T Fujimoto, Y Watanabe, T Niki, H Suzushima, T Hattori
   Tohoku J. Exp. Med, 2017;241(3):201-208.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


3. Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE
   Authors: D Zhao, M Guo, B Liu, Q Lin, T Xie, Q Zhang, X Jia, Q Shu, X Liang, L Gao, C Ma
   J. Leukoc. Biol., 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Secretion of IFN-? Associated with Galectin-9 Production by Pleural Fluid Cells from a Patient with Extrapulmonary Tuberculosis
   Authors: J Zhao, B Shiratori, H Chagan-Yas, M Matsumoto, T Niki, M Tanaka, Y Takahashi, O Usami, Y Ashino, T Hattori
   Int J Mol Sci, 2017;18(7):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


5. Comprehensive analyses of tumor immunity: implications for cancer immunotherapy
   Genome Biol, 2016;17(1):174.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. TIM-1 promotes hepatitis C virus cell attachment and infection
   Authors: Guangxiang Luo
   J. Virol., 2016;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Soluble T cell immunoglobulin mucin domain 3 is shed from CD8+ T cells by the sheddase ADAM10, is increased in plasma during untreated HIV infection, and correlates with HIV disease progression.
   Authors: Clayton K, Douglas-Vail M, Nur-ur Rahman A, Medcalf K, Xie I, Chew G, Tandon R, Lanteri M, Norris P, Deeks S, Ndhlovu L, Ostrowski M
   J Virol, 2015;89(7):3723-36.
   Species: Human
   Sample Type: Cell Culture Supernates
   


8. T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.
   Authors: Clayton K, Haaland M, Douglas-Vail M, Mujib S, Chew G, Ndhlovu L, Ostrowski M
   J Immunol, 2014;192(2):782-91.
   Species: Human
   Sample Type: Cell Lysates
   Application: IP


9. Characterizing functional domains for TIM-mediated enveloped virus entry.
   Authors: Moller-Tank S, Albritton L, Rennert P, Maury W
   J Virol, 2014;88(12):6702-13.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Decreased galectin-9 and increased Tim-3 expression are related to poor prognosis in gastric cancer.
    Authors: Jiang, Jing, Jin, Mei-Shan, Kong, Fei, Cao, Donghui, Ma, Hong-Xi, Jia, Zhifang, Wang, Yin-Ping, Suo, Jian, Cao, Xueyuan
    PLoS ONE, 2013;8(12):e81799.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

目次に戻る