抗Human RAGE抗体(Anti-Human RAGE antibody)
掲載日情報：2021/01/28 現在Webページ番号：30755
Human RAGEに対する抗体(Anti-Human RAGE )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年11月03日 13時35分現在]
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Anti-Human RAGE MAb (Clone 176902) |
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Anti-Human RAGE MAb (Clone 176902) |
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Anti-Human RAGE MAb (Clone 176902) |
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[在庫・価格 ：2025年11月03日 13時35分現在]
Anti-Human RAGE MAb (Clone 176902)
文献数： 12
- 商品コード：MAB11451-100
- メーカー：RSD
- 包装：100μg
- 価格：¥79,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：AGER クローン：176902 Genbank No： 5891 Protein Accession No： Q15109 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | Blocking,ELISA,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human RAGE MAb (Clone 176902)
文献数： 12
- 商品コード：MAB11451-500
- メーカー：RSD
- 包装：500μg
- 価格：¥266,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human RAGE サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF1145，スタンダードとして#1145-RG-050を用いる。 別名：AGER クローン：176902 Genbank No： 5891 Protein Accession No： Q15109 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | Blocking,ELISA,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human RAGE MAb (Clone 176902)
文献数： 12
- 商品コード：MAB11451-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：1個
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：AGER クローン：176902 Genbank No： 5891 Protein Accession No： Q15109 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | Blocking,ELISA,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human RAGEGln24-Ala344Accession # Q15109 |
| Source | Monoclonal Mouse IgG2B Clone # 176902 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human RAGE in ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse RAGE and recombinant rat RAGE is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 2 µg/mL | See below |
| Blockade of Receptor-ligand Interaction | In a functional ELISA, 1-3 μg/mL of this antibody will block 50% of the binding of 500 ng/mL of biotinylated AGE-BSA to immobilized recombinant human RAGE/Fc Chimera coated at 5 μg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding. | |
| Human RAGE Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human RAGE Antibody (Catalog #MAB11451 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human RAGE Biotinylated Antibody (Catalog #BAF1145 ) |
| ELISA Standard | Recombinant Human RAGE Fc Chimera Protein, CF (Catalog #1145-RG ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Western Blot |
![Western Blot RAGE Antibody (176902) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/RAGE_MAB11451_Western_Blot_10850.jpg) click image to view larger | Detection of Human RAGE by Western Blot.Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human RAGE Monoclonal Antibody (Catalog # MAB11451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: RAGE | Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multi-ligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2). The human RAGE gene encodes a 404 amino acid residues (aa) type I transmembrane glycoprotein with a 22 aa signal peptide, a 320 aa extracellular domain containing an Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses and immune or inflammatory disorders (1). |
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References
| Schmidt, A. et al. (2001) J. Clin. Invest. 108:949. | |
| Chavakis, T. et al. (2003) J. Exp. Med. 198:507. | |
| Neeper, M. et al. (1992) J. Biol. Chem. 267:14998. | |
| Yonekura, H. et al. (2003) Biochem. J. 370:1097. | |
| Hori, O. et al. (1995) J. Biol. Chem. 270:25752. | |
| Brett, J. et al. (1993) Am. J. Pathol. 143:1699. | |
| Valencia, J.V. et al. (2004) Diabetes 53:743. | |
| Long Name: | Receptor for Advanced Glycation End Products |
| Entrez Gene IDs: | 177 (Human); 11596 (Mouse); 81722 (Rat) |
| Alternate Names: | AGER; EC 2.7.11.22; MOK Protein Kinase; MOK; RAGE; RAGE-1; Renal tumor antigen 1; renal tumor antigen |
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Related Research Areas
| Adhesion Molecules in Angiogenesis | ||||||||||
| Blood-brain Barrier Transporters | ||||||||||
| Cell Structure and Signaling Molecules | ||||||||||
| Cytokine and Growth Factor Receptors on VSMC | ||||||||||
| Diabetes | ||||||||||
| Diabetic Peripheral Neuropathy | ||||||||||
| Glucose Homeostasis | ||||||||||
| Immune Regulatory Molecules on Endothelial Cells | ||||||||||
| Inflammatory Mediators | ||||||||||
| Integrins and Associated Molecules | ||||||||||
| Neurodegenerative Disease | ||||||||||
| Platelet Immune Receptors | ||||||||||
| ROS and Stress Resistance | ||||||||||
| Uptake of Amyloid beta by Glia Cells | ||||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
 Enlarge | Excellent | ICC/IF | Human | Anonymous | 07/01/2018 | Image Details |
 Immunocytochemistry/Immunofluorescence: Human RAGE Antibody [MAB11451]. | |||
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Summary
| Application | Immunocytochemistry/Immunofluorescence |
| Sample Tested | HUMAN LUNG |
| Species | Human |
| Western Blot | |
|---|---|
 | Detection of Human RAGE by Western Blot. Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human RAGE Monoclonal Antibody (Catalog # MAB11451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
追加しました。
Related Product & Information
| Long Name | Receptor for Advanced Glycation End Products |
|---|---|
| Entrez Gene IDs | 177 (Human); 11596 (Mouse); 81722 (Rat) |
| Background | RAGE |
| background_content | Background: RAGE Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multi-ligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2).The human RAGE gene encodes a 404 amino acid residues (aa) type I transmembrane glycoprotein with a 22 aa signal peptide, a 320 aa extracellular domain containing an Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses and immune or inflammatory disorders (1). |
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Citations
- Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis
Authors: B Zhou, P Flodby, J Luo, DR Castillo, Y Liu, FX Yu, A McConnell, B Varghese, G Li, NO Chimge, M Sunohara, MN Koss, W Elatre, P Conti, JM Liebler, C Yang, CN Marconett, IA Laird-Offr, P Minoo, K Guan, BR Stripp, ED Crandall, Z Borok
J. Clin. Invest., 2018;0(0):.
Species: Mouse
Sample Type: Tissue Homogenates
Application: WB - High-density lipoproteins suppress A?-induced PBMC adhesion to human endothelial cells in bioengineered vessels and in monoculture
Authors: J Robert, EB Button, S Stukas, GK Boyce, E Gibbs, CM Cowan, M Gilmour, WH Cheng, SK Soo, B Yuen, A Bahrabadi, K Kang, I Kulic, G Francis, N Cashman, CL Wellington
Mol Neurodegener, 2017;12(1):60.
Species: Human
Sample Type: Whole Cells
Application: Neut - Autophagy-Lysosome Pathway in Renal Tubular Epithelial Cells Is Disrupted by Advanced Glycation End Products in Diabetic Nephropathy.
Authors: Liu W, Shen T, Chen R, Wu H, Wang Y, Deng J, Chen Q, Pan Q, Huang Fu C, Tao J, Liang D, Liu H
J Biol Chem, 2015;290(33):20499-510.
Species: Human
Sample Type: Whole Cells
Application: Neut - Soluble receptor for advanced glycation end products as an indicator of pulmonary vascular injury after cardiac surgery.
Authors: Tuinman P, Cornet A, Kuipers M, Vlaar A, Schultz M, Beishuizen A, Groeneveld A, Juffermans N
BMC Pulm Med, 2013;13(0):76.
Species: Human
Sample Type: BALF
Application: ELISA Development - Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
Authors: Yamakawa N, Uchida T, Matthay MA, Makita K
Am. J. Physiol. Lung Cell Mol. Physiol., 2011;300(4):L516-25.
Species: Rat
Sample Type: Cell Culture Supernates
Application: ELISA Development - Effects of atorvastatin on serum soluble receptors for advanced glycation end-products in type 2 diabetes.
Authors: Tam HL, Shiu SW, Wong Y, Chow WS, Betteridge DJ, Tan KC
Atherosclerosis, 2010;209(1):173-7.
Species: Human
Sample Type: Cell Lysates
Application: WB - Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
Authors: Zen K, Chen CX, Chen YT, Wilton R, Liu Y
J. Immunol., 2007;178(4):2483-90.
Species: Human
Sample Type: Whole Cells
Application: WB - S100A11, an dual mediator for growth regulation of human keratinocytes.
Authors: Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH
Mol. Biol. Cell, 2007;19(1):78-85.
Species: Human
Sample Type: Cell Lysates
Application: WB
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