抗Galectin-9抗体(Anti-Galectin-9, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human Galectin-9
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Galectin-9 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Galectin-3 and rhGalectin-4 is observed and less than 2% cross-reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-7, and rhGalectin-8 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	0.3-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human Galectin‑9 by Western Blot. Western blot shows conditioned media from HT‑29 human colon adenocarcinoma cell line, human peripheral blood lymphocytes (PBL), and human dendritic cells untreated (-) or treated (+) with 10 µg/mL PHA for 6 days. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Galectin‑9 at approximately 36 and 40 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	Galectin‑9 in Human PBMCs. Galectin‑9 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with Calcium Ionomycin and PMA using Goat Anti-Human Galectin‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
	Galectin‑9 in Human Colon. Galectin‑9 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human Galectin‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Related Product & Information

Entrez Gene IDs	3965 (Human); 16859 (Mouse)
Background	Galectin-9
background_content	Background: Galectin-9 Galectin-9 (also known as Ecalectin and UAT) is a 40 kDa beta -galactosidase binding lectin that belongs to a tandem repeat group of Galectins. Activated cells such as T cells, PMNs, astrocytes, fibroblasts and endothelial cells can express Galectin-9, but its release requires select MMPs. Human to mouse, there is 69% aa sequence identity for Galectin-9.

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Citations

1. Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen
   Authors: JT Schroeder, AP Bieneman
   J. Immunol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Tim-3 Is Upregulated in NK Cells during Early Pregnancy and Inhibits NK Cytotoxicity toward Trophoblast in Galectin-9 Dependent Pathway.
   Authors: Sun J, Yang M, Ban Y, Gao W, Song B, Wang Y, Zhang Y, Shao Q, Kong B, Qu X
   PLoS ONE, 2016;11(1):e0147186.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


3. Galectin-1, -3 and -9 Expression and Clinical Significance in Squamous Cervical Cancer.
   Authors: Punt S, Thijssen V, Vrolijk J, de Kroon C, Gorter A, Jordanova E
   PLoS ONE, 2015;10(6):e0129119.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells.
   Authors: Leskela S, Serrano A, de la Fuente H, Rodriguez-Munoz A, Ramos-Levi A, Sampedro-Nunez M, Sanchez-Madrid F, Gonzalez-Amaro R, Marazuela M
   PLoS ONE, 2015;10(4):e0123938.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Galectin expression profiling identifies galectin-1 and Galectin-9Delta5 as prognostic factors in stage I/II non-small cell lung cancer.
   Authors: Schulkens I, Heusschen R, van den Boogaart V, van Suylen R, Dingemans A, Griffioen A, Thijssen V
   PLoS ONE, 2014;9(9):e107988.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


6. Human cytomegalovirus upregulates expression of the lectin galectin 9 via induction of beta interferon.
   Authors: McSharry B, Forbes S, Cao J, Avdic S, Machala E, Gottlieb D, Abendroth A, Slobedman B
   J Virol, 2014;88(18):10990-4.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. TIM-3 does not act as a receptor for galectin-9.
   Authors: Leitner, Judith, Rieger, Armin, Pickl, Winfried, Zlabinger, Gerhard, Grabmeier-Pfistershammer, Katharin, Steinberger, Peter
   PLoS Pathog, 2013;9(3):e1003253.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


8. Extended co-expression of inhibitory receptors by human CD8 T-cells depending on differentiation, antigen-specificity and anatomical localization.
   Authors: Baitsch L, Legat A, Barba L, Fuertes Marraco SA, Rivals JP, Baumgaertner P, Christiansen-Jucht C, Bouzourene H, Rimoldi D, Pircher H, Rufer N, Matter M, Michielin O, Speiser DE
   PLoS ONE, 2012;7(2):e30852.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Upregulation of the tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection.
   Authors: Nebbia G, Peppa D, Schurich A, Khanna P, Singh H, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, Chinaleong J, Kennedy P, Maini M
   PLoS ONE, 2012;7(10):e47648.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


10. A crucial role for Kupffer cell-derived galectin-9 in regulation of T cell immunity in hepatitis C infection.
    Authors: Mengshol JA, Golden-Mason L, Arikawa T
    PLoS ONE, 2010;5(3):e9504.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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