抗GATA-6抗体(Anti-GATA-6, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human GATA-6Met1-Thr449Accession # Q92908
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human GATA-6 in direct ELISAs and Western blots.  In direct ELISAs, approximately 5% cross-reactivity with recombinant human GATA-5 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Chromatin Immunoprecipitation (ChIP)	5 µg/5 x 106 cells	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human GATA‑6 by Western Blot. Western blot shows cytoplasmic and nuclear extracts from PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA‑6 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chromatin Immunoprecipitation (ChIP)
	Detection of GATA‑6-regulated Genes by Chromatin Immunoprecipitation. KATO‑III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA‑6/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The mucin4 promoter was detected by standard PCR.
Immunocytochemistry
	GATA‑6 in KATO-III Human Cell Line. GATA‑6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	GATA‑6 in Human Intestine. GATA‑6 was detected in immersion fixed paraffin-embedded sections of human intestine using Goat Anti-Human GATA‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Entrez Gene IDs	2627 (Human)
Background	GATA-6
background_content	Background: GATA-6 GATA-6 (also named GATA-GT1) is a zinc finger transcription activating protein that bins to a T/A-G-A-T-A-G/A DNA sequence motif in mesodermally and endodermally-derived tissue. There are two GATA-6 isoforms that arise from alternate start sites: a short form of 449 amino acids (aa), and a long form of 595 aa. The short form of human GATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat and mouse, respectively.

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Citations

1. OCT4/POU5F1 is required for NANOG expression in bovine blastocysts
   Authors: K Simmet, V Zakhartche, J Philippou-, H Blum, N Klymiuk, E Wolf
   Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
   Species: Bovine
   Sample Type: Whole Tissue
   Application: IHC


2. Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency
   Authors: A Molotkov, P Mazot, JR Brewer, RM Cinalli, P Soriano
   Dev. Cell, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


3. Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State
   Authors: X Li, D Liu, Y Ma, X Du, J Jing, L Wang, B Xie, D Sun, S Sun, X Jin, X Zhang, T Zhao, J Guan, Z Yi, W Lai, P Zheng, Z Huang, Y Chang, Z Chai, J Xu, H Deng
   Cell Stem Cell, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


4. Lineage Establishment and Progression within the Inner Cell Mass of the Mouse Blastocyst Requires FGFR1 and FGFR2
   Authors: M Kang, V Garg, AK Hadjantona
   Dev. Cell, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


5. The chromatin modifier Satb1 regulates cell fate through Fgf signalling in the early mouse embryo
   Authors: M Goolam, M Zernicka-G
   Development, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


6. ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation
   Authors: S Bessonnard, S Coqueran, S Vandormael, A Dufour, J Artus, M Cohen-Tann
   Sci Rep, 2017;7(1):12285.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Gata6 promotes hair follicle progenitor cell renewal by genome maintenance during proliferation
   Authors: Alex B Wang
   EMBO J, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


8. p38 (Mapk14/11) occupies a regulatory node governing entry into primitive endoderm differentiation during preimplantation mouse embryo development
   Open Biol, 2016;6(9):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Fluorescent


9. Asynchronous fate decisions by single cells collectively ensure consistent lineage composition in the mouse blastocyst
   Nat Commun, 2016;7(0):13463.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Fresh


10. Abrogation Of Gap Junctional Communication In Es Cells Results In A Disruption Of Primitive Endoderm Formation In Embryoid Bodies
    Stem Cells, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Fresh

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