抗Human Mer抗体(Anti-Human Mer antibody)

掲載日情報:2021/01/28 現在Webページ番号:30546

Human Merに対する抗体(Anti-Human Mer )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2024年05月04日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-Human Mer MAb (Clone 125518)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
別名:c-Eyk
クローン:125518
Genbank No: 10461
Protein Accession No: AAB60430
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Mouse クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド
Anti-Human Mer MAb (Clone 125518)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
マッチドペア:Human Mer サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF891,スタンダードとして#891-MR-100を用いる。
別名:c-Eyk
クローン:125518
Genbank No: 10461
Protein Accession No: AAB60430
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Mouse クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド
Anti-Human Mer MAb (Clone 125518)
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
※受注発注品。形状:溶液または凍結乾燥
別名:c-Eyk
クローン:125518
Genbank No: 10461
Protein Accession No: AAB60430
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Mouse クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

[在庫・価格 :2024年05月04日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Human Mer MAb (Clone 125518)

文献数: 0

説明文 別名:c-Eyk
クローン:125518
Genbank No: 10461
Protein Accession No: AAB60430
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Mouse
交差性 Human 適用 ELISA,FCM
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

Anti-Human Mer MAb (Clone 125518)

文献数: 0

説明文 マッチドペア:Human Mer サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF891,スタンダードとして#891-MR-100を用いる。
別名:c-Eyk
クローン:125518
Genbank No: 10461
Protein Accession No: AAB60430
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Mouse
交差性 Human 適用 ELISA,FCM
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

Anti-Human Mer MAb (Clone 125518)

文献数: 0

説明文 ※受注発注品。形状:溶液または凍結乾燥
別名:c-Eyk
クローン:125518
Genbank No: 10461
Protein Accession No: AAB60430
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Mouse
交差性 Human 適用 ELISA,FCM
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド



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Product Details

Species ReactivityHuman
LabelUnconjugated
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human MerMet1-Ala499Accession # AAB60430
SourceMonoclonal Mouse IgG2B Clone # 125518
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects human Mer in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Axl, rhDtk, or recombinant mouse Mer is observed.


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Applications and Data

 Recommended
Concentration
Sample
Flow Cytometry0.25 µg/106 cellsSee below
CyTOF-readyReady to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Human Mer Sandwich ImmunoassayReagent
ELISA Capture (Matched Antibody Pair)2-8 µg/mL Human Mer Antibody (Catalog #MAB8912 )
ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mL Human Mer Biotinylated Antibody (Catalog #BAF891 )
ELISA Standard  Recombinant Human Mer Fc Chimera Protein, CF (Catalog #891-MR )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Flow Cytometry Flow Cytometry Mer Antibody (125518) [Unconjugated]
click image to view larger
Detection of Mer in HepG2 Human Cell Line by Flow Cytometry.HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Flow Cytometry Flow Cytometry Mer Antibody (125518) [Unconjugated]
click image to view larger
Detection of Mer in U937 Human Cell Line by Flow Cytometry.U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.Reconstitution Buffer Available
ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.

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References

Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name:Receptor Tyrosine Protein Kinase Mer
Entrez Gene IDs:10461 (Human); 17289 (Mouse)
Alternate Names:c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer

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Related Research Areas

ShowShow
Cancer Biomarkers
Infectious Diseases
Mouse Tissue-Resident Macrophage Markers
Receptor Tyrosine Kinases (RTKs)
Receptor Tyrosine Kinases (RTKs) in the Akt Pathway
Receptors in the Jak/STAT Pathway
  1. CD14(+)CD15(-)HLA-DR(-) myeloid-derived suppressor cells impair antimicrobial responses in patients with acute-on-chronic liver failure
    Authors: C Bernsmeier, E Triantafyl, R Brenig, FJ Lebosse, A Singanayag, VC Patel, OT Pop, W Khamri, R Nathwani, R Tidswell, CJ Weston, DH Adams, MR Thursz, JA Wendon, CG Antoniades
    Gut, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
ImagesRatingsApplicationsSpeciesReviewed ByDateDetails
Very Good
  Anonymous 04/26/2017
Functional Assay Human Mer Antibody MAB8912 Array MAB8912
Enlarge
Excellent
 Functional Assay Human Anonymous 06/07/2016 Image Details Functional Assay Human Mer Antibody MAB8912
Functional Assay: Human Mer Antibody [MAB8912].

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Summary

Application Functional Assay
Sample Tested NCI-H1299
Species Human


Flow Cytometry
Detection of Mer in HepG2 Human Cell Line by Flow Cytometry.
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Flow Cytometry
Detection of Mer in U937 Human Cell Line by Flow Cytometry.
U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).


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Related Product & Information

BackgroundMer
background_contentBackground:
Mer
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
  1. CD14(+)CD15(-)HLA-DR(-) myeloid-derived suppressor cells impair antimicrobial responses in patients with acute-on-chronic liver failure
    Authors: C Bernsmeier, E Triantafyl, R Brenig, FJ Lebosse, A Singanayag, VC Patel, OT Pop, W Khamri, R Nathwani, R Tidswell, CJ Weston, DH Adams, MR Thursz, JA Wendon, CG Antoniades
    Gut, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

  2. The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles
    Authors: KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck
    J. Biol. Chem., 2016;291(20):10586-601.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

  3. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
    J. Exp. Med., 2012;209(11):2033-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

  4. Expression of the vitamin K-dependent proteins GAS6 and protein S and the TAM receptor tyrosine kinases in human atherosclerotic carotid plaques.
    Authors: Hurtado B, Munoz X, Recarte-Pelz P, Garcia N, Luque A, Krupinski J, Sala N, Garcia de Frutos P
    Thromb. Haemost., 2011;105(5):873-82.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB

  5. GAS6/Mer axis regulates the homing and survival of the E2A/PBX1-positive B-cell precursor acute lymphoblastic leukemia in the bone marrow niche.
    Authors: Shiozawa Y, Pedersen EA, Taichman RS
    Exp. Hematol., 2010;38(2):132-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow



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